Aldosterone Human ELISA (Multispecies specificity)

Research topic

Animal studies, Steroid hormones

Summary

Aldosterone is a potent mineral corticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension. Measurement of aldosterone levels in serum in conjunction with plasma renin levels can be used to differentiate between primary and secondary aldosteronism.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

50 μl

Applications

Serum, Urine

Calibration Curve

Calibration range

0 – 2000 pg/ml

Limit of detection

15 pg/ml

Intra-assay

CV = 6.5%

Inter-assay

CV = 8.7%

Spiking Recovery

106.5 %

Dilution Linearity

97.1 %

References to summary

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• Sequeira SJ, Loughlin T, Cunningham S, Culliton MT, Hannon S, Heffernan A, McKenna TJ. Evaluation of an aldosterone radioimmunoassay: the renin-angiotensin-aldosterone axis as a function of sex and age. Ann Clin Biochem. 1986 Jan;23 ( Pt 1):65-75
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• Al-Dujaili EA, Edwards CR. Optimization of a direct radioimmunoassay for plasma aldosterone. J Steroid Biochem. 1981 May;14 (5):481-7
• Himathongkam T, Dluhy RG, Williams GH. Potassim-aldosterone-renin interrelationships. J Clin Endocrinol Metab. 1975 Jul;41 (1):153-9
• Vallotton MB. Primary aldosteronism. Part I. Diagnosis of primary hyperaldosteronism. Clin Endocrinol (Oxf). 1996 Jul;45 (1):47-52
• Corry DB, Tuck ML. Secondary aldosteronism. Endocrinol Metab Clin North Am. 1995 Sep;24 (3):511-29