Alpha-1-Antitrypsin in Stool Human ELISA

Summary

Alpha-1-antitrypsin (α-1-AT) is a 52 kDa glycoprotein, which is produced by the liver, intestinal macrophages, monocytes and mucous membrane cells of the gut. It belongs to the group of acute phase proteins and is one of the most important proteinase inhibitor. α-1-AT inhibits, beside others, the proteinases trypsin and the elastase of neutrophiles. A lack of α-1-AT leads to an enhanced proteolysis. Only a very small amount of α-1-AT is cleaved or resorbed in the gut. Therefore the measurement of α-1-AT in stool reflects the permeability of the gut during inflammatory processes.

Research topic

Immune Response, Infection and Inflammation

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Stool

Sample requirements

100 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Limit of detection

1.5 ng/ml

Spiking Recovery

18.75 ng/ml

By research topic:

• Immune Response, Infection and Inflammation

By molecule:

• Alpha-1-Antitrypsin