Androstenedione Human ELISA (Multispecies specificity)

Research topic

Animal studies, Steroid hormones

Summary

Androstenedione is produced by the adrenals and gonads. As a result, the determination of the level of androstenedione in serum is important in the evaluation of the functional state of these glands. Androstenedione is a precursor of testosterone and estrone. Besides the adrenals, in females, the ovaries have been shown to be an important source of androstenedione. It has been reported that there is a fluctuation day by day of androstenedione during the ovulatory cycle. The use of serum testosterone determination in conjunction with the enzyme immunoassay of androstenedione can be used to determine if the source of the excess androgen production is adrenal or ovarian.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

25 μl

Applications

Serum

Calibration Curve

Calibration range

0 – 10 ng/ml

Limit of detection

0.02 ng/ml

References to summary

• Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40