Angiotensin-Converting Enzyme (ACE) Human ELISA

Summary

Angiotensin-converting enzyme (ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073.1 Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm.2 The standard product used in this kit is recombinant human ACE, consisting of 30–1261 amino acids with the molecular mass of 120KDa.

Research topic

Blood pressure regulation and NO metabolism, Renal disease

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Body fluids, Cell culture and/or animal studies, Plasma, Serum, Tissue lysates

Storage/Shipping

Store at 4°C for frequent use, at –20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Calibration Curve

Calibration range

156 pg/ml-10 000 pg/ml

Limit of detection

< 5 pg/ml

By research topic:

• Blood pressure regulation and NO metabolism
• Renal disease

By molecule:

• Angiotensin-Converting Enzyme (ACE)

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