Annexin V Human ELISA

Summary

Annexin belongs to calcium-dependent phospholipid-binding proteins. All annexins have been shown to have putative binding site for protein kinases C but only annexin V would possess a potential pseudo-substrate site. Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel.

Research topic

Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

Store kit reagents between 2 – 8°C.

Calibration Curve

Calibration range

0.8 – 50 ng/ml

Limit of detection

0.33 ng/ml

Intra-assay

CV = 10 %

Inter-assay

CV = 11 %

Spiking Recovery

85 %

Dilution Linearity

89 %

References to summary

• Center DM, Berman JS, Kornfeld H, Theodore AC, Cruikshank WW. The lymphocyte chemoattractant factor. J Lab Clin Med. 1995 Feb;125 (2):167-72
• Center DM, Cruikshank W. Modulation of lymphocyte migration by human lymphokines. I. Identification and characterization of chemoattractant activity for lymphocytes from mitogen-stimulated mononuclear cells. J Immunol. 1982 Jun;128 (6):2563-8
• Center DM, Cruikshank WW, Berman JS, Beer DJ. Functional characteristics of histamine receptor-bearing mononuclear cells. I. Selective production of lymphocyte chemoattractant lymphokines with histamine used as a ligand. J Immunol. 1983 Oct;131 (4):1854-9
• Cruikshank WW, Berman JS, Theodore AC, Bernardo J, Center DM. Lymphokine activation of T4+ T lymphocytes and monocytes. J Immunol. 1987 Jun 1;138 (11):3817-23

By research topic:

• Autoimmunity

By molecule:

• Annexin V

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