Anti-Annexin V Human ELISA

Summary

Annexin belongs to calcium-dependent phospholipid-binding proteins. All annexins have been shown to have putative binding site for protein kinases C but only annexin V would possess a potential pseudo-substrate site. Annexin V was found to play a major role in matrix vesicle-initiated cartilage calcification as a collage-regulated calcium channel.

Research topic

Autoimmunity

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl/well

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

6.25 to 400 ng/ml

Limit of detection

1.18 ng/ml

Intra-assay

CV = 2.0%

Inter-assay

CV = 3.8%

Spiking Recovery

86 %

Dilution Linearity

107 %

References to summary

• Rodriguez-Garcia MI, Fernandez JA, Rodriguez A, Fernandez MP, Gutierrez C, Torre-Alonso JC. Annexin V autoantibodies in rheumatoid arthritis. Ann Rheum Dis. 1996 Dec;55 (12):895-900
• von der Mark K, Mollenhauer J. Annexin V interactions with collagen. Cell Mol Life Sci. 1997 Jun;53 (6):539-45
• Reutelingsperger CP, van Heerde WL. Annexin V, the regulator of phosphatidylserine-catalyzed inflammation and coagulation during apoptosis. Cell Mol Life Sci. 1997 Jun;53 (6):527-32
• Huber R, Berendes R, Burger A, Luecke H, Karshikov A. Annexin V-crystal structure and its implications on function. Behring Inst Mitt. 1992 Apr; (91):107-25
• Dubois T, Oudinet JP, Mira JP, Russo-Marie F. Annexins and protein kinases C. Biochim Biophys Acta. 1996 Oct 11;1313 (3):290-4
• Benz J, Hofmann A. Annexins: from structure to function. Biol Chem. 1997 Mar-Apr;378 (3-4):177-83
• Matsuda J, Gotoh M, Saitoh N, Gohchi K, Tsukamoto M, Yamamoto T. Anti-annexin antibody in the sera of patients with habitual fetal loss or preeclampsia. Thromb Res. 1994 Jul 1;75 (1):105-6
• Kuragaki C, Kidoguchi K, Nakamura N, Wada Y. Anti-annexin V antibodies in plasma and serum samples from patients with lupus anticoagulant. Am J Hematol. 1995 Sep;50 (1):68
• Matsuda J, Saitoh N, Gohchi K, Gotoh M, Tsukamoto M. Anti-annexin V antibody in systemic lupus erythematosus patients with lupus anticoagulant and/or anticardiolipin antibody. Am J Hematol. 1994 Sep;47 (1):56-8
• Nakamura N, Kuragaki C, Shidara Y, Yamaji K, Wada Y. Antibody to annexin V has anti-phospholipid and lupus anticoagulant properties. Am J Hematol. 1995 Aug;49 (4):347-8
• Kaburaki J, Kuwana M, Yamamoto M, Kawai S, Ikeda Y. Clinical significance of anti-annexin V antibodies in patients with systemic lupus erythematosus. Am J Hematol. 1997 Mar;54 (3):209-13
• Nakamura N, Shidara Y, Kawaguchi N, Azuma C, Mitsuda N, Onishi S, Yamaji K, Wada Y. Lupus anticoagulant autoantibody induces apoptosis in umbilical vein endothelial cells: involvement of annexin V. Biochem Biophys Res Commun. 1994 Dec 15;205 (2):1488-93
• Galli M. Non beta 2-glycoprotein I cofactors for antiphospholipid antibodies. Lupus. 1996 Oct;5 (5):388-92
• Kaburaki J, Ikeda Y. [Thrombosis in patients with SLE and antiphospholipid syndrome]. Rinsho Ketsueki. 1995 Apr;36 (4):320-4

By research topic:

• Autoimmunity

By molecule:

• anti-Annexin V