Anti-IgA isotype IgG Human ELISA

Summary

Anti-IgA in the IgG class are specific IgG antibodies against immunoglobulin A. Patients with IgA deficiency often demonstrate circulating antibodies against IgA, which have been suggested to be associated with transfusion reactions. Anti-IgA antibodies occur in about 10% of patients with selective IgA deficiency (IgAD) and are detected approximately in the same percentage of patients with Common Variable Immunodeficiency (CVID). Patients with anti-IgA antibodies are at risk to develop an anaphylactoid reaction to substitution gamma globulin treatment, especially upon intravenous administration.

Features

• It is intended for research use only.
• The total assay time is less than 3 hours.
• The kit measures IgG anti-IgA antibodies in serum and plasma (EDTA, citrate, heparin).
• Assay format is 96 wells.
• Calibrator is serum based.
• Quality Controls are human serum based. No animal sera are used.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Autoimmunity, Immune Response, Infection and Inflammation, Transplantation

Find out more on biovendor.com

• Screening of IgG anti-IgA antibodies in hypogammaglobulinemic subjects with specific readily available IgG anti-IgA ELISA test may prevent anaphylactoid reactions in BioVendor News

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma, Serum

Sample requirements

5 μl/well

Storage/Shipping

2 – 8° C

Calibration Curve

Calibration range

1.56 – 100 U/ml

Limit of detection

0.71 U/ml

Intra-assay (Within-Run, n=8)

CV = 4.8 %

Inter-assay (Run-to-Run, n=5)

CV = 5.3 %

Spiking Recovery

102%

Dilution Linearity

91%

Cross-Reactivity

References to this product

• Chovancova Z, Vlkova M, Litzman J, Lokaj J, Thon V. Antibody forming cells and plasmablasts in peripheral blood in CVID patients after vaccination. Vaccine. 2011 Apr 5;

References to summary

• . An enzyme immunoassay for the determination of anti-IgA antibodies using polyclonal human IgA. J Immunol Methods. 1995 Feb 13;179 (1):51-8
• . An enzyme-linked immunosorbent assay applicable to screen blood donors for IgA deficiency. Haematologica. 1999 Oct;84 (10):887-90
• . Anti-IgA antibodies in common variable immunodeficiency (CVID): diagnostic workup and therapeutic strategy. Clin Immunol. 2007 Feb;122 (2):156-62
• . Anti-IgA in selective IgA deficiency. In vitro effects and Ig subclass pattern of human anti-IgA. Scand J Immunol. 1983 Dec;18 (6):509-13
• . Genetic linkage of IgA deficiency to the major histocompatibility complex: evidence for allele segregation distortion, parent-of-origin penetrance differences, and the role of anti-IgA antibodies in disease predisposition. Am J Hum Genet. 1999 Apr;64 (4):1096-109
• . Hemagglutination assays for the diagnosis and prevention of IgA anaphylactic transfusion reactions. Blood. 1994 Sep 15;84 (6):2031-5
• . Hematology. Mayo Clinic Internal Medicin. 2008; (Eight Edition):427
• . IgA deficiency in Czech healthy individuals and selected patient groups. Int Arch Allergy Immunol. 2000 Oct;123 (2):177-80
• . IgG anti-IgA subclasses in common variable immunodeficiency and association with severe adverse reactions to intravenous immunoglobulin therapy. J Clin Immunol. 2000 Jan;20 (1):77-82
• . Induction of unresponsiveness against IgA in IgA-deficient patients on subcutaneous immunoglobulin infusion therapy. Clin Exp Immunol. 1998 May;112 (2):341-6
• . Rapid detection of antibodies to immunoglobulin A molecules by using the particle gel immunoassay. Vox Sang. 2001 Jul;81 (1):45-8
• . Review: IgA anaphylactic transfusion reactions. Part I. Laboratory diagnosis, incidence, and supply of IgA-deficient products. Immunohematology. 2004;20 (4):226-33
• . Review: IgA anaphylactic transfusion reactions. Part II. Clinical diagnosis and bedside management. Immunohematology. 2004;20 (4):234-8
• . Safe and effective use of chromatographically purified intravenous immunoglobulin despite profound anti-IgA sensitization. Intern Med J. 2001 May-Jun;31 (4):256-7
• . Transfusion medicine. In Henry's Clinical Diagnosis . 2006;:707-708
• . [IgA deficiency]. Arch Pediatr. 2001;8 (6):629-33
• Davenport RD, Mintz PD. Transfusion medicine. In Henry's Clinical Diagnosis . 2006;:707-708
• de Albuquerque Campos R, Sato MN, da Silva Duarte AJ. IgG anti-IgA subclasses in common variable immunodeficiency and association with severe adverse reactions to intravenous immunoglobulin therapy. J Clin Immunol. 2000 Jan;20 (1):77-82
• Hammarstrom L, Persson MA, Smith CI. Anti-IgA in selective IgA deficiency. In vitro effects and Ig subclass pattern of human anti-IgA. Scand J Immunol. 1983 Dec;18 (6):509-13
• Horn J, Thon V, Bartonkova D, Salzer U, Warnatz K, Schlesier M, Peter HH, Grimbacher B. Anti-IgA antibodies in common variable immunodeficiency (CVID): diagnostic workup and therapeutic strategy. Clin Immunol. 2007 Feb;122 (2):156-62
• Koskinen S, Hirvonen M, Tolo H. An enzyme immunoassay for the determination of anti-IgA antibodies using polyclonal human IgA. J Immunol Methods. 1995 Feb 13;179 (1):51-8
• Limaye S, Walls RS, Riminton S. Safe and effective use of chromatographically purified intravenous immunoglobulin despite profound anti-IgA sensitization. Intern Med J. 2001 May-Jun;31 (4):256-7
• Litzman J, Sevcikova I, Stikarovska D, Pikulova Z, Pazdirkova A, Lokaj J. IgA deficiency in Czech healthy individuals and selected patient groups. Int Arch Allergy Immunol. 2000 Oct;123 (2):177-80
• Quartier P. [IgA deficiency]. Arch Pediatr. 2001;8 (6):629-33
• Salama A, Schwind P, Schonhage K, Genth R, Cotting C, Hustinx H, Krieg R, Nydegger U, Aebischer I. Rapid detection of antibodies to immunoglobulin A molecules by using the particle gel immunoassay. Vox Sang. 2001 Jul;81 (1):45-8
• Sandler SG, Eckrich R, Malamut D, Mallory D. Hemagglutination assays for the diagnosis and prevention of IgA anaphylactic transfusion reactions. Blood. 1994 Sep 15;84 (6):2031-5
• Sandler SG, Zantek ND. Review: IgA anaphylactic transfusion reactions. Part II. Clinical diagnosis and bedside management. Immunohematology. 2004;20 (4):234-8
• Sanz C, Freire C, Ordinas A, Pereira A. An enzyme-linked immunosorbent assay applicable to screen blood donors for IgA deficiency. Haematologica. 1999 Oct;84 (10):887-90
• Steensma DP, Pruthi RK. Hematology. In Mayo Clinic Internal Medici. 2008; (Eight Edition):427
• Sundin U, Nava S, Hammarstrom L. Induction of unresponsiveness against IgA in IgA-deficient patients on subcutaneous immunoglobulin infusion therapy. Clin Exp Immunol. 1998 May;112 (2):341-6
• Vassallo RR. Review: IgA anaphylactic transfusion reactions. Part I. Laboratory diagnosis, incidence, and supply of IgA-deficient products. Immunohematology. 2004;20 (4):226-33
• Vorechovsky I, Webster AD, Plebani A, Hammarstrom L. Genetic linkage of IgA deficiency to the major histocompatibility complex: evidence for allele segregation distortion, parent-of-origin penetrance differences, and the role of anti-IgA antibodies in disease predisposition. Am J Hum Genet. 1999 Apr;64 (4):1096-109

By research topic:

• Autoimmunity
• Immune Response, Infection and Inflammation
• Transplantation

By molecule:

• Anti-IgA IgG

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