APRIL Human ELISA

Summary

APRIL is a member of the tumor necrosis factor family. APRIL appears to play a role in T-independent type II antigen responses and T cell survival, but can also induce proliferation/sur­vival of nonlymphoid cells. Local production of APRIL was found in arthritic joints of patients with inflammatory arthritis.

Research topic

Apoptosis, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.78 – 50 ng/ml

Limit of detection

0.4 ng/ml

Intra-assay

CV = 8.1%

Inter-assay

CV = 7.1%

Spiking Recovery

76%

Dilution Linearity

113%

References to summary

• Ashkenazi A, Grewal IS, Varfolomeev E, Kischkel F, Martin F, Seshasayee D, Wang H, Lawrence D, Olsson C, Tom L, Erickson S, French D, Schow P. APRIL-deficient mice have normal immune system development. Mol Cell Biol. 2004 Feb;24 (3):997-1006
• Cerutti A, Litinskiy MB, Nardelli B, Hilbert DM, He B, Schaffer A, Casali P. DCs induce CD40-independent immunoglobulin class switching through BLyS and APRIL. Nat Immunol. 2002 Sep;3 (9):822-9
• Hahne M, Kataoka T, Schroter M, Hofmann K, Irmler M, Bodmer JL, Schneider P, Bornand T, Holler N, French LE, Sordat B, Rimoldi D, Tschopp J. APRIL, a new ligand of the tumor necrosis factor family, stimulates tumor cell growth. J Exp Med. 1998 Sep 21;188 (6):1185-90
• Hahne M, Lopez-Fraga M, Fernandez R, Albar JP. Biologically active APRIL is secreted following intracellular processing in the Golgi apparatus by furin convertase. EMBO Rep. 2001 Oct;2 (10):945-51
• Hardenberg G, Hahne M, Planelles-Carazo L, Medema JP. The uncertain glory of APRIL. Cell Death Differ. 2003 Oct;10 (10):1121-5
• Kelly K, Manos E, Jensen G, Nadauld L, Jones DA. APRIL/TRDL-1, a tumor necrosis factor-like ligand, stimulates cell death. Cancer Res. 2000 Feb 15;60 (4):1021-7
• Mackay F, Ambrose C. The TNF family members BAFF and APRIL: the growing complexity. Cytokine Growth Factor Rev. 2003 Jun-Aug;14 (3-4):311-24
• Mackay F, Schneider P, Rennert P, Browning J. BAFF AND APRIL: a tutorial on B cell survival. Annu Rev Immunol. 2003;21:231-64
• MacLennan I, Vinuesa C. Dendritic cells, BAFF, and APRIL: innate players in adaptive antibody responses. Immunity. 2002 Sep;17 (3):235-8
• Marsters SA, Yan M, Pitti RM, Haas PE, Dixit VM, Ashkenazi A. Interaction of the TNF homologues BLyS and APRIL with the TNF receptor homologues BCMA and TACI. Curr Biol. 2000 Jun 29;10 (13):785-8
• Migone TS, Roschke V, Sosnovtseva S, Ward CD, Hong JS, Smith R, Albert V, Stohl W, Baker KP, Ullrich S, Nardelli B, Hilbert DM. BLyS and APRIL form biologically active heterotrimers that are expressed in patients with systemic immune-based rheumatic diseases. J Immunol. 2002 Oct 15;169 (8):4314-21
• Patel DR, Wallweber HJ, Yin J, Shriver SK, Marsters SA, Gordon NC, Starovasnik MA, Kelley RF. Engineering an APRIL-specific B cell maturation antigen. J Biol Chem. 2004 Apr 16;279 (16):16727-35
• Rennert P, Schneider P, Cachero TG, Thompson J, Trabach L, Hertig S, Holler N, Qian F, Mullen C, Strauch K, Browning JL, Ambrose C, Tschopp J. A soluble form of B cell maturation antigen, a receptor for the tumor necrosis factor family member APRIL, inhibits tumor cell growth. J Exp Med. 2000 Dec 4;192 (11):1677-84
• Stohl W, Tan SM, Xu D, Roschke V, Perry JW, Arkfeld DG, Ehresmann GR, Migone TS, Hilbert DM. Local production of B lymphocyte stimulator protein and APRIL in arthritic joints of patients with inflammatory arthritis. Arthritis Rheum. 2003 Apr;48 (4):982-92
• Theill LE, Yu G, Boone T, Delaney J, Hawkins N, Kelley M, Ramakrishnan M, McCabe S, Qiu WR, Kornuc M, Xia XZ, Guo J, Stolina M, Boyle WJ, Sarosi I, Hsu H, Senaldi G. APRIL and TALL-I and receptors BCMA and TACI: system for regulating humoral immunity. Nat Immunol. 2000 Sep;1 (3):252-6
• Weller M, Roth W, Wagenknecht B, Klumpp A, Naumann U, Hahne M, Tschopp J. APRIL, a new member of the tumor necrosis factor family, modulates death ligand-induced apoptosis. Cell Death Differ. 2001 Apr;8 (4):403-10
• Xu S, Lam KP. B-cell maturation protein, which binds the tumor necrosis factor family members BAFF and APRIL, is dispensable for humoral immune responses. Mol Cell Biol. 2001 Jun;21 (12):4067-74

By research topic:

• Apoptosis
• Cytokines and chemokines and related molecules

By molecule:

• APRIL

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