Bcl-2 Human ELISA

Summary

Bcl-2 initiates a new gene family involved in the regulation of cell death and survival without affecting cell proliferation. Bcl-2 protein plays a critical role in oncogenesis as well as resistance to therapy for cancer.

Research topic

Apoptosis

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture lysate, Plasma, Serum

Sample requirements

20 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.5 – 32 ng/ml

Limit of detection

0.5 ng/ml

Intra-assay

CV = 8.6%

Inter-assay

CV = 12%

Spiking Recovery

71%

Dilution Linearity

102%

References to summary

• Andreeff M, Savchenko V, Goodacre A, Munker R, Snell V, Sanchez-Williams G, Consoli U, Zhang X, Estrov Z, Lisovsky M. Flt3 ligand stimulates proliferation and inhibits apoptosis of acute myeloid leukemia cells: regulation of Bcl-2 and Bax. Blood. 1996 Nov 15;88 (10):3987-97
• Cohen IR, Mor F. IL-2 rescues antigen-specific T cells from radiation or dexamethasone-induced apoptosis. Correlation with induction of Bcl-2. J Immunol. 1996 Jan 15;156 (2):515-22
• Gabbert HE, Willers R, Dutkowski P, Verreet P, Porschen R, Bittinger F, Sarbia M. bcl-2 expression and prognosis in squamous-cell carcinomas of the esophagus. Int J Cancer. 1996 Aug 22;69 (4):324-8
• Hanada M, Kitada S, Bodrug S, Aime-Sempe C, Krajewski S, Sato T, Wang HG, Takayama S, Miyashita T, Reed JC. BCL-2 family proteins: regulators of cell death involved in the pathogenesis of cancer and resistance to therapy. J Cell Biochem. 1996 Jan;60 (1):23-32
• Harris A, Dalla Palma P, Doglioni C, Pezzella F, Eccher C, Morelli L, Girlando S, Bonzanini M, Fox S, Fina P, Leek RD, Veronese S, Caffo O, Barbareschi M. Bcl-2 and p53 expression in node-negative breast carcinoma: a study with long-term follow-up. Hum Pathol. 1996 Nov;27 (11):1149-55
• Kaushansky K, Yee E, Karsan A, Harlan JM. Cloning of human Bcl-2 homologue: inflammatory cytokines induce human A1 in cultured endothelial cells. Blood. 1996 Apr 15;87 (8):3089-96
• Lalani EN, Foster CS, Abel P, Lu QL. bcl-2: role in epithelial differentiation and oncogenesis. Hum Pathol. 1996 Feb;27 (2):102-10
• Linch DC, Selby P, Mason D, Vaughan Hudson G, Anderson L, Di Stefano F, Clarke P, Burke M, Vaughan Hudson B, Cunningham DC, MacLennan KA, Hill ME. Prognostic significance of BCL-2 expression and bcl-2 major breakpoint region rearrangement in diffuse large cell non-Hodgkin's lymphoma: a British National Lymphoma Investigation Study. Blood. 1996 Aug 1;88 (3):1046-51
• Miyazaki K, Tokunaga O, Sasatomi E. Spontaneous apoptosis in gallbladder carcinoma. Relationships with clinicopathologic factors, expression of E-cadherin, bcl-2 protooncogene, and p53 oncosuppressor gene. Cancer. 1996 Nov 15;78 (10):2101-10
• Park JR, Hockenbery DM. BCL-2, a novel regulator of apoptosis. J Cell Biochem. 1996 Jan;60 (1):12-7
• Pileri A, Boccadoro M, Bianchi A, Montacchini L, Beggiato E, Barral P, Attisano C, Borrione P, Massaia M. Dysregulated Fas and Bcl-2 expression leading to enhanced apoptosis in T cells of multiple myeloma patients. Blood. 1995 Jun 15;85 (12):3679-87
• Su IJ, Jin YT, Yan JJ, Chen FF. Detection of bcl-2 and p53 in thymoma: expression of bcl-2 as a reliable marker of tumor aggressiveness. Hum Pathol. 1996 Oct;27 (10):1089-92

By research topic:

• Apoptosis

By molecule:

• Bcl-2

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