Beta2-Microglobulin Rat ELISA

Summary

ß2-microglobulin occurs in blood, urine, and body fluids. It has been reported that its concentration in blood varies in humans under pathological conditions such as glomerular disturbances, autoimmune diseases, malignant tumors and hepatic disturbances, while its concentration in urine is subject to changes by urethral disturbances and by the changes in its concentration in blood.

Research topic

Animal studies, Renal disease

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum, Urine

Sample requirements

10 µl/well

Storage/Shipping

Stability is assured until the demonstrated expiration date (one year after manufacture), following storage in the dark and cool place (2–10°C).

Calibration Curve

Calibration range

0.31 to 10 ng/ml

References to summary

• Brodsky FM, Bodmer WF, Parham P. Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979 Jul;9 (7):536-45
• Burnett RW. Accurate estimation of standard deviations for quantitative methods used in clinical chemistry. Clin Chem. 1975 Dec;21 (13):1935-8
• Cadafalch J, Ris J, Sambeat MA, Cortes M, Fuster M, Martinez C, Martinez E, Domingo P. Prognostic value of serum beta 2-microglobulin in HIV infection. Lancet. 1992 Aug 8;340 (8815):371-2
• Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Engvall E, Ruoslahti E, Uotila M. Two-site sandwich enzyme immunoassay with monoclonal antibodies to human alpha-fetoprotein. J Immunol Methods. 1981;42 (1):11-5
• Evrin PE, Jansson NO, Bergdahl A. A turbidimetric immunochemical method for determination of serum beta 2-microglobulin using a centrifugal analyzer. Clin Chim Acta. 1986 Mar 16;155 (2):151-7
• Fahey JL, Taylor JM, Detels R, Hofmann B, Melmed R, Nishanian P, Giorgi JV. The prognostic value of cellular and serologic markers in infection with human immunodeficiency virus type 1. N Engl J Med. 1990 Jan 18;322 (3):166-72
• Hansson LO, Strannegard OO, von Stedingk LV, Sonnerborg AB. Elevated neopterin and beta 2-microglobulin levels in blood and cerebrospinal fluid occur early in HIV-1 infection. AIDS. 1989 May;3 (5):277-83
• Lacey JN, Forbes MA, Waugh MA, Cooper EH, Hambling MH. Serum beta 2-microglobulin and human immunodeficiency virus infection. AIDS. 1987 Jul;1 (2):123-7
• Lauer CC, Ubelacker L, Check JH. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• LeClair K, Van Agthoven A, Terhorst C. A rapid method for isolation of antigenically active human cell surface antigens associated with beta 2-microglobulin using a monoclonal antibody. J Immunol Methods. 1981;41 (2):137-44
• Mattiasson B, Ling TG. A general study of the binding and separation in partition affinity ligand assay. Immunoassay of beta 2-microglobulin. J Immunol Methods. 1983 May 13;59 (3):327-37
• O'Reilly D, Sanderson AR, Whicher J. A simple, precise and sensitive nephelometric assay for beta 2-microglobulin in body fluids. J Immunol Methods. 1983 Feb 25;57 (1-3):265-73
• Ponten J, Berggard I, Evrin PE, Nilsson K. Involvement of lymphoid and nonlymphoid cells in the production of beta2-microglobulin-a homologue of the constant domains of IgG. Nat New Biol. 1973 Jul 11;244 (132):44-5
• Schwartz MK, Mertelsmann R, Safai B, Bhalla RB. Abnormally high concentrations of beta 2 microglobulin in acquired immunodeficiency syndrome (AIDS) patients. Clin Chem. 1983 Aug;29 (8):1560
• Tachibana K, Tanaka E, Usuda S, Okamoto H, Imai M, Nakamura T, Miyakawa Y, Mayumi M. A two-site sandwich radioimmunoassay of beta 2-microglobulin with monoclonal antibodies. J Immunol Methods. 1984 Dec 14;75 (1):43-51
• Vaudois CH, Clement F, Francioli P. Beta 2-microglobulin and immunodeficiency in a homosexual man. N Engl J Med. 1982 Nov 25;307 (22):1402-3
• Wibell L, Evrin PE, Berggard I. Serum 2 -microglobulin in renal disease. Nephron. 1973;10 (5):320-31

By research topic:

• Animal studies
• Renal disease

By molecule:

• Beta2-Microglobulin