c-Met Assay Kit-MKI Human ELISA

Research topic

Cytokines and chemokines and related molecules, Oncology

Storage/Shipping

All reagents should be stored at 2–8°C. All reagents shall be brought to room temperature approximately 30 minutes before use.

Summary

c-Met, the product of c-met gene has been reported to be the HGF receptor since HGF induces phosphorylation of c-Met by binding specifically to it. It is a heterodimer protein composed of an –chain that is linked to a /-chain. The /-chain has the intracellular tyrosine kinase domain, the transmembrane domain and the extracellular domain, and it is tied into the –chain that is the extracellular domain of c-Met. c-Met exists mainly in epidermal cells. It is found in the digestive tract, prostate gland, seminal vesicles, mammary gland, microglia cells, monocytes and macrophages, and more amount in the liver and kidney. It is considered that c-Met tansmits signals resulting from binding to HGF, such as growth, motility and organ formation, in the organs and cells mentioned above.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

25 µl/well

Applications

Cell culture medium, Plasma-EDTA, Serum

Calibration Curve

Calibration range

0.78 to 50 ng/ml

Limit of detection

0.26 ng/ml

Intra-assay (Within-Run, n=24)

CV = 7.4 %

Inter-assay (Run-to-Run, n=24)

CV = 5.4 %

References to summary

• Kataoka H, Hamasuna R, Itoh H, Kitamura N, Koono M: Activation of hepatocyte growth factor/scatter factor in colorectal carcinoma.Cancer Res. 2000 Nov 1;60(21):6148–59.
• Shimomura T, Denda K, Kitamura A, Kawaguchi T, Kito M, Kondo J, Kagaya S, Qin L, Takata H, Miyazawa K, Kitamura N: Hepatocyte growth factor activator inhibitor, a novel Kunitz-type serine protease inhibitor.J Biol Chem. 1997 Mar 7;272(10):6370–6.
• Kawaguchi T, Qin L, Shimomura T, Kondo J, Matsumoto K, Denda K, Kitamura N: Purification and cloning of hepatocyte growth factor activator inhibitor type 2, a Kunitz-type serine protease inhibitor.J Biol Chem. 1997 Oct 31;272(44):27558–64.