C-peptide (U-type) Mouse ELISA

Summary

C-peptide is a peptide which is made when proinsulin is split into insulin and C-peptide. It exerts beneficial therapeutic effects on may complications associated with diabetes mellitus, such as for instance diabetic neuropathy and other diabetes-induced ailments. C-peptide functions in repair of the muscular layer of the arteries.

Research topic

Animal studies, Diabetology - Insulin, C-Peptide, Proinsulin

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture medium, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

10 µl/well

Storage/Shipping

Store the kit at 2–8°C. Do not freeze.

Calibration Curve

Calibration range

30 to 3 000 pg/ml

Limit of detection

1.5 pg/ml

References to this product

• Guo W, Miao C, Liu S, Qiu Z, Li J, Duan E. Efficient differentiation of insulin-producing cells from skin-derived stem cells. Cell Prolif. 2009 Feb;42 (1):49-62
• Hori Y, Fukumoto M, Kuroda Y. Enrichment of putative pancreatic progenitor cells from mice by sorting for prominin1 (CD133) and platelet-derived growth factor receptor beta. Stem Cells. 2008 Nov;26 (11):2912-20
• Ibii T, Shimada H, Miura S, Fukuma E, Sato H, Iwata H. Possibility of insulin-producing cells derived from mouse embryonic stem cells for diabetes treatment. J Biosci Bioeng. 2007 Feb;103 (2):140-6
• Liu MJ, Shin S, Li N, Shigihara T, Lee YS, Yoon JW, Jun HS. Prolonged remission of diabetes by regeneration of beta cells in diabetic mice treated with recombinant adenoviral vector expressing glucagon-like peptide-1. Mol Ther. 2007 Jan;15 (1):86-93
• Narushima M, Kobayashi N, Okitsu T, Tanaka Y, Li SA, Chen Y, Miki A, Tanaka K, Nakaji S, Takei K, Gutierrez AS, Rivas-Carrillo JD, Navarro-Alvarez N, Jun HS, Westerman KA, Noguchi H, Lakey JR, Leboulch P, Tanaka N, Yoon JW. A human beta-cell line for transplantation therapy to control type 1 diabetes. Nat Biotechnol. 2005 Oct;23 (10):1274-82
• Torisu T, Sato N, Yoshiga D, Kobayashi T, Yoshioka T, Mori H, Iida M, Yoshimura A. The dual function of hepatic SOCS3 in insulin resistance in vivo. Genes Cells. 2007 Feb;12 (2):143-54

By research topic:

• Animal studies
• Diabetology - Insulin, C-Peptide, Proinsulin

By molecule:

• C-peptide