C-Reactive Protein Canine ELISA

Research topic

Animal studies, Infection and inflammation

Storage/Shipping

+4°C/ Wet ice

Summary

C-reactive protein is a plasma protein, a member of the pentraxin family of proteins. It is synthesized in the liver following tissue damage caused by inflammation, infection, or trauma. CRP is considered to be an important acute phase marker in such conditions.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

5 µl/well

Applications

Serum

Calibration Curve

Calibration range

1.5 to 40.0 µg/ml

Limit of detection

3.6 ng/ml

Intra-assay

CV = 3.3 %

Inter-assay

CV = 2.3 %

Spiking Recovery

1 800 ng/ml

References to summary

• Burton, S.A., et. al.: C-reactive protein concentration in dogs with inflammatory leukograms.Am. J.Vet. Res. 1994; 55:613 – 618.
• Casals, C. et. al.: Increase of C-reactive protein and decrease of surfactant protein A in surfactant after lung transplantati­on.Am. J. Respir. Crit. Care. Med. 1998; 157: 43 – 49.
• Conner, J.G., et. al.: Acute phase response in the dog following surgical trauma.Res. Vet. Sci. 1988; 45: 107 – 110.
• Eckersall, P.D. et. al.: An immunoturbidimetric assay for canine C-reactive protein.Vet. Res. Commun. 1991; 15: 17 – 24.
• Lindback, S., et. al.: The value of C-reactive protein as a marker of bacterial infection in patients with septicemia, endocarditis, and influenza.Scand. J. Infect. Dis. 1989; 21: 543 – 549.
• Ndung’u, J.M. et. al.: Elevation of the concentration of acute phase proteins in dogs infected with Trypanosoma brucei.Acta. Trop. 1991;49: 77 – 86.
• Otabe, K.: Physiological levels of C-reactive protein in normal canine sera.Vet. Res. Commun. 1998; 22: 77 – 85.
• Rikihisa, Y. et. al.: C-reactive protein and alpha1-acid glycoprotein levels in dogs infected with Ehrlichia canis.J. Clin. Microbiol. 1994; 32: 912 – 917.
• Yamamoto, S. et. al.:Determination of C-reactive protein in serum and plasma from healthy dogs and dogs with pneumonia by ELISA and slide reversed passive latex agglutination test.Vet. Q. 1994; 16: 74 – 77.
• Yamashita,K., et.al.: Canine acute phase response: relationship between serum cytokine activity and acute phase protein in dogs.J. Vet. Med. Sci. 1994; 56: 487 – 492.