C-Reactive Protein Human ELISA

Research topic

Animal studies, Infection and inflammation

Storage/Shipping

+4°C/ Wet ice

Summary

C-reactive protein is an acute-phase protein produced by the liver in conditions of inflammation, bacterial infection, or tissue trauma. Quantification of CRP is useful in determining inflammatory conditions difficult to diagnose and to monitor the patients’ response to treatment. C-reactive protein is synthesized in the liver following tissue damage caused by inflammation, infection, or trauma. It is considered to be an important acute phase marker in such conditions.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

5µl/well

Applications

Serum

Calibration Curve

Calibration range

0.12 to 10.0 ng/ml

Limit of detection

0.20 ng/ml

Intra-assay (Within-Run, n=12)

CV = 3.8 %

Inter-assay (Run-to-Run, n=12)

CV = 4.8 %

Spiking Recovery

800 ng/ml

References to summary

• Danesh J. et.al.: C-Reactive Protein and Other Circulating Markers of Inflammation in the Prediction of Coronary Heart Disease.N. Engl. J. Med. 2004; 350: 1387 – 1397
• Rifai N. and Ridker, P.: C-Reactive Protein, A New and Strong Predictor of Cardiovascular Disease.Clin. Lab. News. 2001; 27: 12–14.
• Pepys M.B. and Hirschfield G.M.: C-Reactive Protein: A Critical Update.J. Clin. Invest. 2003; 111 :1805–12.
• Volanakis J.E.: Human C-reactive Protein: Expression, Structure, and Function.Mol. Immunol. 2001; 38:189–97.
• Chen, K. et.: Induction of Leptin Resistance Through Direct Interaction of C-reactive Protein with Leptin.Nature Medicine 2006;12:425 – 432