C-Reactive Protein Mouse ELISA

Research topic

Animal studies, Infection and inflammation

Storage/Shipping

C-reactive protein kit components should be stored at 2–8°C. Bring them to room temperature (20–25°C) before opening bottles and plate pouches. Diluted conjugate remaining after use should be discarded. TMB substrate and stop solution are also stable at room temperature.

Summary

C-reactive protein is a plasma protein, a member of the pentraxin family of proteins. It is synthesized in the liver following tissue damage caused by inflammation, infection, or trauma. CRP is considered to be an important acute phase marker in such conditions.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

5µl/well

Applications

Fluids, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Calibration Curve

Calibration range

1.56 to 25.0 ng/ml

Limit of detection

2.5 ng/ml

Intra-assay (Within-Run, n=12)

CV = 4 %

Inter-assay (Run-to-Run, n=12)

CV = 6 %

Spiking Recovery

50 ng/ml

Cross-Reactivity

Rat CRP 100 %