C-Reactive Protein Rat ELISA

Research topic

Animal studies, Infection and inflammation

Storage/Shipping

C-reactive protein kit components should be stored at 2–8°C. Bring them to room temperature (20–25°C) before opening bottles and plate pouches. Diluted conjugate remaining after use should be discarded. TMB substrate and stop solution are also stable at room temperature.

Summary

C-reactive protein is a plasma protein, a member of the pentraxin family of proteins. It is synthesized in the liver following tissue damage caused by inflammation, infection, or trauma. CRP is considered to be an important acute phase marker in such conditions.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

5 µl/well

Applications

Serum

Calibration Curve

Calibration range

1.6 to 133.3 ng/ml

Limit of detection

2.5 ng/ml

Intra-assay (Within-Run, n=12)

CV = 5 %

Inter-assay (Run-to-Run, n=12)

CV = 5 %

Spiking Recovery

10 000 ng/ml

Cross-Reactivity

Mouse

References to summary

• Buduneli, E. et. al.: Systemic Low-Dose Doxycycline and Alendronate Administration and Serum Interleukin-1Beta, Osteocalcin, and C-Reactive Protein Levels in Rats.Journal of Periodontology 2005;76:1927–1933
• Cai, X. et.al.: The comparative study of Sprague–Dawley and Lewis rats in adjuvant-induced arthritis.Arch. Pharmacol. 2006;373:140–147
• Chen, K. et.: Induction of Leptin Resistance Through Direct Interaction of C-reactive Protein with Leptin.Nature Medicine 2006; 12:425 – 432 (Human CRP)
• Cho W. C. et.al.: Differential Expression of Proteins in Kidney, Eye, Aorta, and Serum of Diabetic and Non-diabetic Rats.J. Cell. Biochem. 2006; 99, 256 – 268
• Kalani, R., et.al.: Effects of Caloric Restriction and Exercise on Age-Related, Chronic Inflammation Assessed by C-Reactive Protein and Interleukin-6Effects of Caloric Restriction and Exercise on Age-Related, Chronic Inflammation Assessed by C-Reactive Protein and Interleukin-6.Journal of Gerontology: 2006; 61A: 3, 211–217
• Ling, S. and F. Jabali: Effect of Early Phase Adjuvant Arthritis on Hepatic P450 Enzymes and Pharmacokinetics of Verapamil: An Alternative Approach to the Use of An Animal Model for Inflammation for Pharmacokinetic Studies.Drug Metabolism and Disposition 2005; 33:579–586
• Ortiz, R. et. al.: Urinary C-Reactive Protein Excretion Increases in Angiotensin II-Dependent Hypertensive Rats.FASEB Journal 2006; 20: 470.16
• Shagdarsuren, E. et al.: Complement Activation in Angiotensin II–Induced Organ Damage.Circ Res. 2005; 97:716–724