Cardiotrophin-1 Human ELISA

Research topic

Cardiovascular disease

Features

• The total assay time is less than four hours.
• The kit measures total Cardiotrophin-1.
• Calibrator is recombinat protein based.
• Quality Controls are recombinant protein based.

Storage/Shipping

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Summary

Cardiotrophin-1 (CT-1) is a 201 amino acid member of the interleukin-6 superfamily. It was identified by its ability to induce hypertrophic response in cardiac myocytes. CT-1 mRNA levels were found both in cardiac myocytes and in cardiac nonmyocytes. CT-1 was also detected in abundance in normal adult human lung and was expressed in both fetal and adult airway smooth muscle cells. CT-1activates gp130 dependent signaling and stimulates the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway to transduce hypertrophic and cytoprotective signals in cardiac myocytes. CT-1 has also a neurotrophic function. CT-1 deficiency causes increased motoneuron cell death in spinal cord and brainstem nuclei of mice during a period between embryonic day 14 and the first postnatal week.

Moreover, CT-1 is a hepatocyte survival factor that efficiently reduces hepatocellular damage in animal models of acute liver injury. CT-1 expression is augmented after hypoxic stimulation and it can protect cardiac cells when added either prior to simulated ischaemia or at the time of reoxygenation following simulated ischaemia. CT-1 can induce expression of the protective heat shock proteins (hsps) in cardiac cells. Cardiotrophin-1 increased ventricular expression of ANP, brain natriuretic peptide (BNP) and angiotensinogen mRNA. CT-1 levels were significantly elevated in patients with heart failure, patients with dilatative cardiomyopathy, moderate/severe mitral regurgitation, stable and unstable angina and after acute myocardial infarction.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

50 µl/well

Applications

Plasma-EDTA

Calibration Curve

Calibration range

5 pg/ml to 1 ng/ml

Limit of detection

Analytical Limit of Detection is calculated from the real cardiotrophin-1 values in wells and is 1pg/ml

Limit of quantification

Assay Sensitivity takes the dilution of samples into consideration and is calculated according to the formula: Assay Sensitivity = Analytical Limit of Detection x sample dilution = 1pg/ml x 2 = 2 pg/ml

Intra-assay (Within-Run, n=8)

CV = 5.9 %

Inter-assay (Run-to-Run, n=8)

CV = 5.9 %

Spiking Recovery

92.3 %

Dilution Linearity

89.7 %

Cross-Reactivity

Human

References to summary

• Gard AL, Gavin E, Solodushko V and Pennica D: Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. Neuroscience, 127(1):43–52 (2004)
• Jougasaki M, Leskinen H, Larsen AM, Luchner A, Cataliotti A, Tachibana I and Burnett JC: Ventricular cardiotrophin-1 activation precedes BNP in experimental heart failure. Peptides, Jun;24(6):889–892 (2003)
• Talwar S, Squire IB, O'brien RJ, Downie PF, Davies JE, Ng LL: Plasma cardiotrophin-1 following acute myocardial infarction: relationship with left ventricular systolic dysfunction. Clin Sci (Lond) Jan;102(1):9–14 (2002)
• Bristow MR, Long CS: Cardiotrophin-1 in heart failure: Circulation Sep;106(12):1430–2 (2002)
• Asai S, Saito Y, Kuwahara K, Mizuno Y, Yoshimura M, Higashikubo C, Tsuji T, Kishimoto I, Harada M, Hamanaka I, Takahashi N, Yasue H, Nakao K: The heart is a source of circulating cardiotrophin-1 in humans. Biochem Biophys Res Commun Dec;279(2):320–3 (2000)
• Ghosh S, Ng LL, Talwar S, Squire IB, Galiñanes M: Cardiotrophin-1 protects the human myocardium from ischemic injury. Comparison with the first and second window of protection by ischemic preconditioning. Cardiovasc Res Dec;48(3):440–7 (2000)