Caspase-9 Human ELISA

Summary

Caspase-9 is essentially localized in mitochondria, and the disruption of the outer mitochondrial membrane occurring early during apoptosis is critical for its subcellular redistribution and activation.It plays a crucial role in all pathologies involving apoptotic processes. Specifically, the involvement of caspase-9 has been described in human gastric cancer, ovarian carcinoma, neuroblastoma, gliomas as well as in Alzheimer´s di­sease, Huntington´s di­sease and osteoarthritis.

Research topic

Apoptosis, Immune Response, Infection and Inflammation

Assay format

Sandwich ELISA, 2nd HRP-labelled antibody

Applications

Cell culture lysate, Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

1.6 – 100 ng/ml

Limit of detection

0.4 ng/ml

Intra-assay

CV = 6.6%

Inter-assay

CV = 9.0%

Spiking Recovery

102.7%

Dilution Linearity

102.8%

By research topic:

• Apoptosis
• Immune Response, Infection and Inflammation

By molecule:

• Caspase-9

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