Cathepsin L Human ELISA

Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Lipoprotein metabolism, Oncology

Storage/Shipping

2–8°C

Summary

Cathepsin L is a lysosomal endopeptidase belonging to the papain cysteine protease superfamily. Elevated levels of cathepsin L have been found in primary cell cultures from cancerous prostatic samples and in synovial lining from rheumatoid arthritis and osteoarthritis samples. Cathepsin L was found to be necessary for Li (invariant chain) degradation, a critical step in major histocompatibility complex class II – restricted antigen presentation, in cortical thymic epithelial cells, but not in bone marrow derived antigen – presenting cells.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 µl

Applications

Cell culture supernatant, Serum

Calibration Curve

Calibration range

3.12 – 50 ng/ml

Limit of detection

1.71 ng/ml

Limit of quantification

3.42 ng/ml

Intra-assay

CV = 6.2%

Inter-assay

6.0%

Spiking Recovery

92 %

Dilution Linearity

106.2%