Chromogranin A Human ELISA

Research topic

Oncology

Storage/Shipping

At 4℃ in dark.

Summary

Chromogranin A is an acidic secretory protein consisting of 439 amino acids. The protein is found in a wide variety of hormone and neurotransmitter storage vesicles, and it is known be co-stored and co-relased with catecholamines from adrenal medulla and sympathetic neuronal vesicles during exocytosis.

Assay format

Competitive ELISA, Immobilized antibody

Sample requirements

25 µl/well

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Saliva, Urine

Calibration Curve

Calibration range

10 to 1 000 fmol/ml

Intra-assay

Plasma sample: 10.13 – 13.26 %, Saliva sample: 8.15–12.84 %

Inter-assay

Plasma sample: 11.57–15.33 %, Saliva sample: 12.42 – 14.22 %

Spiking Recovery

Plasma sample: 126.02 %, Saliva sample: 96.6 %

References to summary

• Blaschko H., Comiline R. S., Schneider F. H., Silver M. and Smith A. D.: Secretion of a chromaffin granule protein, chromogranin, from the adrenal gland after splanchnic stimulation.Nature 215, 58–59 (1967)
• Fischer-Colbrie R., Lassmann H., Hang C. and Winkler H.: Immunological studies on the distribution of chromogranin A and B in endocrine and nervous tissues.Neuros­cience 16, 547–55 (1985)
• Konnecki D. S., Benedum U. M., Gerdesh H-H. and Huttner W. B.: The primary structure of human chromogranin A and pancreastatin.J. Biol. Chem. 262, 17025–17030 (1987)
• Mouland A. J., Bevan S., White J. H. and Hendy G. N.: Human chromogranin A gene. Molecular cloning, structural analysis, and neuroendocrine cell-specific expression.J. Biol. Chem. 269, 6918–6926 (1994)
• Nishikawa Y., Li J., Futai Y., Yanaihara N., Iguchi K., Mochizuki T., Hoshino M. and Yanaihara C.: Region-specific radioimmunoassay for human chromogranin A.Biomed. Res. 19, 245–251(1998)
• O'Connor D. T.: Chromogranin : widespread immunoreactivity in polypeptide hormone producing tissues and in serum.Regul. Pept. 6, 263–280 (1983)
• Yanaihara N., Nishikawa Y., Hoshino M., Mochizuki T., Iguchi K., Nagasawa S., Li J., Futai Y., Kanno T., Yanaihara H., Murai M. and Yanaihara C.: Evaluation of region-specific radioimmunoassays for rat and human chromogranin A : measurement of immunoreactivity in plasma, urine and saliva. In The Adrenal Chromaffin Cell: Archetype and Exemplar od Cellular Signalling in Secretory Control (ed. Kanno T., Nakazato Y. and Kumakura K.)Hokkaido University Press, Sapporo, Japan, pp. 305–313 (1998)
• Wu J. T., Astill M. E., Liu G. H. and Stephenson R. A.: Serum chromogranin A : Early detection of hormonal resistance in prostate cancer patients.J. Clin. Lab. Anal. 12, 20–25 (1998)