Corticosterone Human ELISA (Multispecies specificity)

Summary

Corticosterone is a glucocorticoid hormone secreted from the cortex of adrenal gland. Corticosterone is derived from cholesterol through a series of enzymatically mediated steps and also serving as a precursor of aldosterone. It is a primary glucocorticoid in mice and rats and other animals (such as rabbits, birds, amphibians, and reptilians) in which the 17-hydroxylase is supposed not to exist in adrenal gland. Corticosterone is produced under the control of ACTH and the production has a circadian rhythm with peak levels in the latter portion of the day in nocturnal animals like rats and is believed to play a decisive role in sleep-wake cycles. Corticosterone can be used as a non-invasive biomarker of stress study through the collection of urine and feces to avoid corticosterone increase of blood levels which is caused by normal invasive methods. Corticosterone is also being studied in different fields such as impairment of long-term memory retrieval, chronic corticosterone elevation due to dietary restrictions and response to burn injuries etc.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antibody

Applications

Cell culture supernatant, Plasma, Serum, Urine

Storage/Shipping

2 – 8 ℃

Calibration Curve

Calibration range

0.21 – 50 ng/ml

Limit of detection

0.21 – 50 ng/ml

References to summary

• Hupe JM, James AC, Payne BR, Lomber SG, Girard P, Bullier J. Cortical feedback improves discrimination between figure and background by V1, V2 and V3 neurons. Nature. 1998 Aug 20;394 (6695):784-7
• Kitaysky AS, Kitaiskaia EV, Wingfield JC, Piatt JF. Dietary restriction causes chronic elevation of corticosterone and enhances stress response in red-legged kittiwake chicks. J Comp Physiol B. 2001 Nov;171 (8):701-9
• Thellin O, Noel G, Khurana S, Ogle CK, Horseman ND. Stress hormone secretion and gut signal transducer (STAT) proteins after burn injury in rats. Shock. 2001 Nov;16 (5):393-7
• Velazquez-Moctezuma J, Vazquez-Palacios G, Retana-Marquez S, Bonilla-Jaime H. Further definition of the effect of corticosterone on the sleep-wake pattern in the male rat. Pharmacol Biochem Behav. 2001 Oct-Nov;70 (2-3):305-10

By research topic:

• Steroid hormones

By molecule:

• Corticosterone