Corticotropin Releasing Factor ELISA Mouse/Rat, Sensitive, Extraction-free

Research topic

Animal studies

Storage/Shipping

Store all of the components at 2–8°C.

Summary

CRF is a 41-amino acid polypeptide hormone and neurotransmitter involved in the stress response. It is produced by neuroendocrine cells in the paraventricular nucleus of the hypothalamus and is released from neurosecretory terminals of these neurons into the primary capillary plexus of the hypothalamo-hypophyseal portal system. The portal system carries the CRH to the anterior lobe of the pituitary, where it stimulates corticotropes to secrete corticotropin and other biologically active substances.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

50 µl/well

Applications

Brain extract, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin

Calibration Curve

Calibration range

0.078 to 2.5 ng/ml

References to summary

• Vale W, Spiess J, Rivier C, Rivier J: Characterization of a 41-residue ovine hypothalamic peptide at stimulates secretion of corticotropin and beta-endorphin.Science, 213, 1394–1397, 1981
• Shibahara S, Morimoto Y, Furutani Y, Notake M, Takahashi H, Shimizu S, Horikawa S, Numa S: Isolation and sequence analysis of the human corticotropin-releasing factor precursor gene.EMBO J, 2, 775–779, 1983
• Rivier J, Spiess J, Vale W: Characterization of rat hypothalamic corticotropin-releasing factor.Proc Natl Acad Sci USA, 80, 4851–4855, 1983
• Seasholtz AF, Bourbonais FJ, Harnden CE, and Camper SA: Nucleotide Sequence and Expression of the Mouse Corticotropin-Releasing Hormone Gene.Mol Cell Neurosci, 2, 266–273, 1991
• Nakane T, Audhya T, Hollander CS, Schlesinger DH, Kardos P, Brown C, Passarelli J: Corticotrophin-releasing factor in extra-hypothalamic brain of the mouse: demonstration by immunoassay and immunoneutralizaion of bioassayable activity.J Endocrinol, 111, 143–149, 1986
• Chappell PB, Smith MA, Kilts CD, Bissette G, Ritchie J, Anderson C, Nemeroff CB: Alterations in corticotropin-releasing factor-like immunoreactivity in discrete rat brain regions after acute and chronic stress.J Neurosci, 6, 2908–2914, 1986
• Ur E, Grossman A: Corticotropin-releasing hormone in health and disease: an update.Acta Endocrinol (Copenh), 127, 193–199. Review, 1992
• Geracioti TD Jr, Orth DN, Ekhator NN, Blumenkopf B, Loosen PT: Serial cerebrospinal fluid corticotropin-releasing hormone concentrations in healthy and depressed humans.J Clin Endocrinol Metab, 74, 1325–1330, 1992
• May C, Rapoport SI, Tomai TP, Chrousos GP, Gold PW: Cerebrospinal fluid concentrations of corticotropin-releasing hormone (CRH) and corticotropin (ACTH) are reduced in patients with Alzheimer's di­sease.Neurolo­gy, 37, 535–538, 1987
• Davis KL, Mohs RC, Marin DB, Purohit DP, Perl DP, Lantz M, Austin G, Haroutunian V: Neuropeptide abnormalities in patients with early Alzheimer disease.Arch Gen Psychiatry, 56, 981–987, 1999
• Behan DP, Khongsaly O, Owens MJ, Chung HD, Nemeroff CB, De Souza EB: Corticotropin-releasing factor (CRF), CRF-binding protein (CRF-BP), and CRF/CRF-BP complex in Alzheimer's disease and control postmortem human brain.J Neurochem, 68, 2053–2060, 1997
• Behan DP, Heinrichs SC, Troncoso JC, Liu XJ, Kawas CH, Ling N, De Souza EB: Displacement of corticotropin releasing factor from its binding protein as a possible treatment for Alzheimer's di­sease.Nature, 378, 284–287, 1995
• Pedersen WA, McCullers D, Culmsee C, Haughey NJ, Herman JP, Mattson MP: Corticotropin-releasing hormone protects neurons against insults relevant to the pathogenesis of Alzheimer's di­sease.Neurobi­ol Dis, 8, 492–503, 2001
• Tsuchihashi T, Yamaguchi K, Abe K, Yanaihara N, Saito S: Production of immunoreactive corticotropin-releasing hormone in various neuroendocrine tumors.Jpn J Clin Oncol, 22, 232–237, 1992