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Cortisol ELISA

Product of BioVendor
Product: Size:
RCAN-C-270R (regulatory status: RUO) 96 wells (1 kit)
Files: Datasheet PDF (RUO)MSDS (RUO) Cortisol on pubmed

Product details


Summary

Cortisol is the most abundant circulating steroid and the major glucocorticoid secreted by the adrenal cortex. Cortisol is physiologically effective in blood pressure maintenance and anti-inflammatory activity. It is also involved in calcium absorption, gluconeogenesis as well as the secretion of gastric acid and pepsin. Measurement of blood cortisol levels can be used as an indicator of adrenal function and the differential diagnosis of Addison's and Cushing's diseases as well as adrenal hyperplasia and carcinoma.

Research topic

Steroid hormones


Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

20 μl/well

Storage/Shipping

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

0.5–60 μg/dl

Limit of detection

0.4 μg/dl

Intra-assay

CV = 5.8%

Inter-assay

CV = 5.6%

Spiking Recovery

109.4%

Dilution Linearity

100.8%


References to summary

  • Brock P, Eldred EW, Woiszwillo JE, Doran M, Schoemaker HJ. Direct solid-phase 125I radioimmunoassay of serum cortisol. Clin Chem. 1978 Sep;24 (9):1595-8
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Demers LM, Derck DD. Comparison of competitive protein binding analysis and radioimmunoassay for the determination of cortisol in serum and urine. Clin Biochem. 1977 Jun;10 (3):104-8
  • Morris R. A simple and economical method for the radioimmunoassay of cortisol in serum. Ann Clin Biochem. 1978 May;15 (3):178-83
  • Poland RE, Rubin RT. Saliva cortisol levels following dexamethasone administration in endogenously depressed patients. Life Sci. 1982 Jan 11;30 (2):177-81
  • Silver AC, Landon J, Smith DS, Perry LA. Radioimmunoassay of cortisol in saliva with the "GammaCoat" kit. Clin Chem. 1983 Oct;29 (10):1869-70
  • Vecsei P, Penke B, Katzy R, Baek L. Radioimmunological determination of plasma cortisol. Experientia. 1972 Sep 15;28 (9):1104-5

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