CRP Human ELISA

Summary

CRP is an acute-phase protein, produced exclusively in the liver. Serum concentration of CRP increases significantly in cases of both infectious and noninfectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours. CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatic arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease. The CRP level increases dramatically following microbial infections, and this can be particularly helpful for the diagnosis and monitoring of bacterial septicemia in neonates and other immunocompromised patients at risk.

Research topic

Immune Response, Infection and Inflammation, Sepsis

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Plasma, Serum

Sample requirements

10 µl/well

Storage/Shipping

2 – 8 °C

Calibration Curve

Calibration range

0 – 100 μg/ml

Limit of detection

1 μg/ml

References to summary

• Gewurz H, Mold C, Siegel J, Fiedel B. C-reactive protein and the acute phase response. Adv Intern Med. 1982;27:345-72
• Helgeson NGP, Adamson DM, Pike RB, James DS, Nicodemus DS, Lee BA, Miller GW. C-Reactive Protein : Laboratory Medicine, Vol. 2 (Race G. J., Ed.). Harper & Row, Hagerstown, chap. 1973;
• Johnson HL, Chiou CC, Cho CT. Applications of acute phase reactants in infectious diseases. J Microbiol Immunol Infect. 1999 Jun;32 (2):73-82
• Powell LJ. C-reactive protein--a review. Am J Med Technol. 1979 Feb;45 (2):138-42

By research topic:

• Immune Response, Infection and Inflammation
• Sepsis

By molecule:

• CRP

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