Cu/Zn Superoxide dismutase Human ELISA

Summary

Superoxide dismutase is the antioxidation enzyme existing in a wide variety of biological systems, and it shows typical antioxidation activity similar to glutathione and ascorbic acid. It plays an important role in inhibiting the negative effects of oxidative stress in living systems. Cu,Zn-SOD detected in cytoplasma has a dimeric structure which consists of subunits having the molecular weight of 32 kDa.

Research topic

Oxidative stress

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Amniotic fluid, Cell culture supernatant, Fetal umbilical vein blood, Plasma, Serum, Urine

Sample requirements

10 µl (1:20 prediluted)

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

0.08 – 5 ng/ml

Limit of detection

0.04 ng/ml

Intra-assay

CV = 5.1%

Inter-assay

CV = 5.8%

Spiking Recovery

98%

Dilution Linearity

90%

By research topic:

• Oxidative stress

By molecule:

• Cu,Zn-Superoxide dismutase

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