Cystatin C Rat ELISA
| Other names: | Post G-globulin, CST 3 | ||
|---|---|---|---|
| Cat. No.: |
 RD391009200R |
Regulatory status: RUO | |
| Size: | 96 wells (1 kit) | | | |
| Files: | Datasheet PDF (RUO) MSDS (RUO) | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
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Research topic
Animal studies, Renal disease
Features
- It is intended for research use only.
- The total assay time is less than 3.5 hours.
- The kit measures total Cystatin C in rat serum.
- Assay format is 96 wells.
- Quality Controls are animal serum based. No human sera are used.
- Standard is recombinant protein based.
- Components of the kit are provided ready to use, concentrated or lyophilized.
Storage/Shipping
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Summary
Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.
Assay format
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum
Calibration range
0.78 – 50 ng/ml
Limit of detection
0.06 ng/ml
Limit of quantification
6 ng/ml
Intra-assay
CV = 5.7%
Inter-assay
CV = 11.6%
Spiking Recovery
98.0%
Dilution Linearity
102.2%
Cross-Reactivity
Hamster, Mouse
References to summary
- Page M.K. et al.: . Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 (2000) .
- Pirttila TJ, Pitkanen A. Cystatin C expression is increased in the hippocampus following photothrombotic stroke in rat. Neurosci Lett. 2006 Mar 6;395(2):108-13. Epub 2005 Nov 23.
- Bokenkamp A, Ciarimboli G, Dieterich C. Cystatin C in a rat model of end-stage renal failure. Ren Fail. 2001 May-Jul;23(3-4):431-8.
- Nagai A, Ryu JK, Kobayash S, Kim SU. Cystatin C induces neuronal cell death in vivo. Ann N Y Acad Sci. 2002 Nov;977:315-21.
- Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C prevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol Dis. 2005 Feb;18(1):152-65.
- Price Ch.P., Finney H. Developments in the assessment of glomerular filtration rate. Clinica Chimica Acta 297, 55-66 (2000) .
- Ono S. et al. Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis.
- Nicklin M.J.H. and Barrett A.J. Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984) .
- Lindahl P., Abrahamson M., Bjork L.: . Interaction of recombinant human cystatin C with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996) .
- Roald AB, Aukland K, Tenstad O. Tubular absorption of filtered cystatin-C in the rat kidney. Exp Physiol. 2004 Nov;89(6):701-7. Epub 2004 Aug 24.
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