Cystatin C Rat ELISA

Summary

Cystatin C is a non-glycosilated basic single-chain protein consisting of 120 amino acids with a molecular weight of 13.36 kDa and is characterized by two disulfide bonds in the carboxy-terminal region. It belongs to the cystatins superfamilly which inactivates lysosomal cysteine proteinases, e.g. cathepsin B, H,.K, L and S. Imbalance between Cystatin C and cysteine proteinases is associated with inflammation, renal failure, cancer, Alzheimer's di­sease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Its increased level has been found in patients with autoimune diseases, with colorectal tumors and in patients on dyalisis. Serum Cystatin C seems to be better marker of glomerular filtration rate than creatinine. On the other hand, low concentration of Cystatin C presents a risk factor for secondary cardiovascular events.

Features

• It is intended for research use only.
• The total assay time is less than 3.5 hours.
• The kit measures total Cystatin C in rat serum.
• Assay format is 96 wells.
• Quality Controls are animal serum based. No human sera are used.
• Standard is recombinant protein based.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Animal studies, Renal disease

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Storage/Shipping

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

0.078 – 25 ng/ml

Limit of detection

0.008 ng/ml

Intra-assay

CV = 4.7%

Inter-assay

CV = 4.2 %

Spiking Recovery

104.7 %

Dilution Linearity

102.8 %

Cross-Reactivity

References to this product

• Tsuda H, Isaka Y, Takahara S, Horio M. Discrepancy between serum levels of low molecular weight proteins in acute kidney injury model rats with bilateral ureteral obstruction and bilateral nephrectomy. Clin Exp Nephrol. 2009 Dec;13 (6):567-70

References to summary

• Bokenkamp A, Ciarimboli G, Dieterich C. Cystatin C in a rat model of end-stage renal failure. Ren Fail. 2001 May-Jul;23(3-4):431-8.
• Lindahl P., Abrahamson M., Bjork L.:. Interaction of recombinant human cystatin C with the cysteine proteinase papain and actinidin. Biochem. J. 281, 49-55 (1996) .
• Nagai A, Ryu JK, Kobayash S, Kim SU. Cystatin C induces neuronal cell death in vivo. Ann N Y Acad Sci. 2002 Nov;977:315-21.
• Nicklin M.J.H. and Barrett A.J. Inhibition of cysteine proteinases and dipeptidyl peptidase 1 by eggwhite cystatin. Biochem J. 223, 245-253 (1984) .
• Ono S. et al. Increased cystatin C immunoreactivity in the skin in amyotrophic lateral sclerosis.
• Page M.K. et al.:. Clinical value of cystatin C determination. Clinica Chim. Acta 297, 67-72 (2000) .
• Pirttila TJ, Pitkanen A. Cystatin C expression is increased in the hippocampus following photothrombotic stroke in rat. Neurosci Lett. 2006 Mar 6;395(2):108-13. Epub 2005 Nov 23.
• Price Ch.P., Finney H. Developments in the assessment of glomerular filtration rate. Clinica Chimica Acta 297, 55-66 (2000) .
• Roald AB, Aukland K, Tenstad O. Tubular absorption of filtered cystatin-C in the rat kidney. Exp Physiol. 2004 Nov;89(6):701-7. Epub 2004 Aug 24.
• Xu L, Sheng J, Tang Z, Wu X, Yu Y, Guo H, Shen Y, Zhou C, Paraoan L, Zhou J. Cystatin C prevents degeneration of rat nigral dopaminergic neurons: in vitro and in vivo studies. Neurobiol Dis. 2005 Feb;18(1):152-65.

By research topic:

• Animal studies
• Renal disease

By molecule:

• Cystatin C

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