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Cytochrome c Human ELISA

Product of BioVendor
Other names: CYC
Cat. No.: new product RBMS263R Regulatory status: RUO
Size: 96 wells (1 kit) |
Files: Datasheet PDF (RUO)
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Notice

Restriction of a minimal quantity of 5 kits per order may apply.

Research topic

Apoptosis, Autoimmunity, Infection and inflammation

Storage/Shipping

Store kit reagents between 2 – 8°C.

Summary

Mitochrondrial cytochrome c (15 kDa) is a water–soluble protein. Cytochrome c functions in the respiratory chain by interacting with redox partners. It is highly conserved during evolution. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps of initiation of apoptosis in cells.

Assay format

Sandwich ELISA, Biotin-labelled antibody

Sample requirements

100 µl

Applications

Cell culture lysate, Serum, Whole blood

Calibration Curve

Calibration range

0.08 – 5 ng/ml

Limit of detection

0.05 ng/ml

Intra-assay

CV = 6.0%

Inter-assay

CV = 3.96%

Spiking Recovery

82%

Dilution Linearity

98%



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