Cytochrome c Human ELISA

Summary

Mitochrondrial cytochrome c (15 kDa) is a water–soluble protein. Cytochrome c functions in the respiratory chain by interacting with redox partners. It is highly conserved during evolution. Measurement of cytochrome c release from the mitochondria is a tool to detect the first early steps of initiation of apoptosis in cells.

Research topic

Apoptosis, Immune Response, Infection and Inflammation, Oxidative stress

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture lysate

Sample requirements

100 µl

Storage/Shipping

Store kit reagents between 2 – 8°C.

Calibration Curve

Calibration range

0.08 – 5 ng/ml

Limit of detection

0.05 ng/ml

Intra-assay

CV = 6.0%

Inter-assay

CV = 3.96%

Spiking Recovery

82%

Dilution Linearity

98%

By research topic:

• Apoptosis
• Immune Response, Infection and Inflammation
• Oxidative stress

By molecule:

• Cytochrome c

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