Dehydroepiandrosterone Sulfate ELISA

Summary

Dehydroepiandros­terone sulfate (DHEAS) is produced by the adrenals and gonads. As a result, the determination of the level of DHEA-S in serum is important in the evaluation of the functional state of these glands. DHEAS is a precursor of testosterone and estrone. Besides the adrenals in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle. An abnormal testosterone level in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with Elisa of DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

25 μl

Calibration Curve

Calibration range

0 – 10 μg/ml

Limit of detection

0.005 μg/ml

Intra-assay

CV = 9.3%

Inter-assay

CV = 9.2%

Spiking Recovery

99.5%

Dilution Linearity

95.7%

By research topic:

• Steroid hormones

By molecule:

• Dehydroepiandrosterone sulfate