Dehydroepiandrosterone Sulfate ELISA

Summary

Dehydroepiandros­terone sulfate (DHEAS) is produced by the adrenals and gonads. As a result, the determination of the level of DHEA-S in serum is important in the evaluation of the functional state of these glands. DHEAS is a precursor of testosterone and estrone. Besides the adrenals in females, the ovaries have been shown to be an important source of DHEAS. It has been reported that there is a fluctuation day by day of DHEAS in women during the ovulatory cycle. An abnormal testosterone level in women should be accompanied by the estimation of serum DHEAS. The use of serum testosterone determination in conjunction with Elisa of DHEAS can be used to determine if the source of excess androgen production is ovarian or adrenal.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

50 μl

Calibration Curve

Calibration range

0 .005– 10 μg/ml

Limit of detection

0.005 μg/ml

Intra-assay

CV = 9.3%

Inter-assay

CV = 9.2%

Spiking Recovery

99.5%

Dilution Linearity

95.7%

References to summary

• Bradlow HL, Blethen SL, Chasalow FI. Dehydroepiandrosterone sulfate (DHEA-S) and DHEA-S-like compounds in fibrocystic disease of the breast. Steroids. 1988 Sep;52 (3):205-15
• Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• de Peretti E, Forest MG. Pattern of plasma dehydroepiandrosterone sulfate levels in humans from birth to adulthood: evidence for testicular production. J Clin Endocrinol Metab. 1978 Sep;47 (3):572-7
• Holtzclaw WD, Gordon GB. Measurement of serum levels of dehydroepiandrosterone sulfate: a comparison of radioimmunoassay and enzymatic analysis. Steroids. 1989 Oct;54 (4):355-71
• Koritnik, D.R., et al. An Elisa for DHEAS in the ciruclation of rhesus monkeys. Steroids. 42/6:653-664, 1984;
• Rizer RL, Vogelman JH, Orentreich N, Brind JL. Age changes and sex differences in serum dehydroepiandrosterone sulfate concentrations throughout adulthood. J Clin Endocrinol Metab. 1984 Sep;59 (3):551-5
• Smith, M.R., et al. An Elisa for the estimation of serum dehydroepiandrosterone sulfate in normal and pathological sera. Clin. Chim. Acta. 65: 5-13, 1975;
• Zeitlin S, Duckett D, Greenfield J, Blethen SL, Chasalow FI. Serum levels of dehydroepiandrosterone sulfate as determined by commercial kits and reagents. Steroids. 1989 Oct;54 (4):373-83

By research topic:

• Steroid hormones

By molecule:

• Dehydroepiandrosterone sulfate

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