Dihydrotestosterone ELISA

Summary

5-dihydrotestosterone (DHT) is a steroid similar to testosterone and androstenedione, which belong to a vlase called androgens. DHT is a C19 steroid and possesses androgenic activity. The bulk of androgen production takes place mainly in the Leydig cells of the testes. Androgens circulate in the blood bound to proteins, especially sex hormone binding globulin (SHBG) and albumin. A trace amount of these steroids circulate in the unbound form in the blood and are referred to as the free fractions. DHT has at least three times the binding affinity for SHBG than testosterone. In males about 70% of DHT is derived from peripheral conversion of testosterone, while in females most of the DHT is derived from androstenedione. The major organ to neutralize androgens is the liver. Therefore in the liver the steroid hormones undergo structural modifications that are generally regarded as prerequisites for their biological inactivation. Some metabolites are formed and some are returned to the circulation

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

50 μl

Calibration Curve

Calibration range

25 – 2500 pg/ml

Limit of detection

6.0 pg/ml

Intra-assay

CV = 6.92%

Inter-assay

CV = 8.5%

Spiking Recovery

90.6%

Dilution Linearity

95.4%

References to summary

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• Jacobs HS, Baxendale PM, James VH. Plasma and salivary androstenedione and dihydrotestosterone in women with hyperandrogenism. Clin Endocrinol (Oxf). 1983 May;18 (5):447-57
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• Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clin Chem. 1999 Jul;45 (7):942-56
• Lauer CC, Ubelacker L, Check JH. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Magrini G, Pellaton M, Mean F. Study on the binding of dihydrotestosterone, testosterone and oestradiol with sex hormone binding globulin. Clin Chim Acta. 1977 Oct 1;80 (1):171-80
• Makin HLJ, Brooks RV. Androgens. Physiology and PathologyIn. Biochemistry of Steroid Hormon. 565:1984.;
• Pazzagli M. Radioimmunoassay of plasma dihydrotestosterone in normal and hypogonadal men. Clin. Endocr. 82:380, 1976;
• Vihko R, Koskela E, Kontturi M, Ruokonen A, Hammond GL. The simultaneous radioimmunoassay of seven steroids in human spermatic and peripheral venous blood. J Clin Endocrinol Metab. 1977 Jul;45 (1):16-24
• Wakelin K. Relationship of 5ßdihydrotestosterone and 5αdihydrotestosterone to testosetrone in health and disease. J. Endocrinol. 87:450, 1980;
• Wood PJ, White J, Wakelin K, Wang C. Salivary androgens in hirsutism: are they of use in routine evaluation?. Ann Clin Biochem. 1986 Sep;23 ( Pt 5):590-5

By research topic:

• Steroid hormones

By molecule:

• Dihydrotestosterone

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