Eotaxin Mouse ELISA

Summary

Eotaxin is a member of the CC chemokine family of inflammatory and immunoregulatory cytokines. Eotaxin expression has been detected in smooth muscle cells, chondrocytes and eosinophils. Mouse Eotaxin has been shown to be a potent chemoattractant for eosinophils during inflammation and allergic reactions, and it is involved in the growth of myeloid cell progenitors and differentiation of mast cells during embryonic development. A role in numerous eosinophil-associated gastrointestinals disorders such as food allergy, parasitic infections, allergic colitis and inflammatory Bowel disease has been described.

Research topic

Animal studies, Immune Response, Infection and Inflammation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

31.3 – 2000 pg/ml

Limit of detection

3.6 pg/ml

Intra-assay

CV = 5.6%

Inter-assay

CV = 9.4%

Spiking Recovery

66%

Dilution Linearity

114%

By research topic:

• Animal studies
• Immune Response, Infection and Inflammation

By molecule:

• Eotaxin