Estradiol Human ELISA (Multispecies specificity)

Research topic

Animal studies, Steroid hormones

Summary

Estradiol is one of the main components of naturally occurring estrogens and is the major estrogen secreted during the menstrual cycle. The serum levels of estradiol are low during the follicular phase rising gradually until about one day before ovulation when a marked rise in the estradiol level occurs (Ovulatory Peak). The estradiol level falls rapidly at, or right after ovulation and is again within the levels of the follicular phase. There is a second rise of estradiol around day 21 of the cycle (Luteal Peak). The levels then decline gradually to the lowest level at the onset of the next menstrual cycle.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

50 μl

Applications

Serum

Calibration Curve

Calibration range

0 – 3200 pg/ml

Limit of detection

10 pg/ml

Intra-assay

CV = 7.7%

Inter-assay

CV = 8.7%

Spiking Recovery

104.1 %

Dilution Linearity

96.5 %