Estrone ELISA

Summary

Estrone is a steroid like estriol and estradiol, belonging to the class of estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the estrone level shows a slight increase.

Research topic

Steroid hormones

Assay format

Competitive ELISA, Immobilized antigen

Applications

Serum

Sample requirements

50 μl

Storage/Shipping

2–8 °C

Calibration Curve

Calibration range

15 – 2000 pg/ml

Limit of detection

10.0 pg/ml

Intra-assay

CV = 7.7%

Inter-assay

CV = 9.5%

Spiking Recovery

99.45%

Dilution Linearity

95.5%

References to summary

• Folan J, Gosling JP, Finn MF, Fottrell PF. Solid-phase enzymoimmunoassay of estrone in saliva. Clin Chem. 1989 Apr;35 (4):569-72
• Fottrell PF, Gosling JP, Folan J. Solid-phase enzymoimmunoassay of estrone in serum. Clin Chem. 1988 Sep;34 (9):1843-6
• Hauptmann H, Paulus B, Kaiser T, Luppa PB. Concepts for the syntheses of biotinylated steroids. Part II: 17beta-estradiol derivatives as immunochemical probes. Bioconjug Chem. 2000 Jul-Aug;11 (4):537-48
• Kim MH, Hosseinian AH, Dupon C. Plasma levels of estrogens, androgens and progesterone during normal and dexamethasone-treated cycles. J Clin Endocrinol Metab. 1974 Oct;39 (4):706-12
• Kricka LJ. Human anti-animal antibody interferences in immunological assays. Clin Chem. 1999 Jul;45 (7):942-56
• Lauer CC, Ubelacker L, Check JH. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
• Meyer HH, Sauerwein H, Mutayoba BM. Immunoaffinity chromatography and a biotin-streptavidin amplified enzymeimmunoassay for sensitive and specific estimation of estradiol-17 beta. J Steroid Biochem. 1990 Feb;35 (2):263-9
• Oakey RE, Hancock KW, Speight AC. Non-protein-bound oestrogens in plasma and urinary excretion of unconjugated oestrogens in non-pregnant women. J Endocrinol. 1979 Dec;83 (3):385-91
• Speroff L et al. Hormone biosynthesis, metabolism, and mechanism of action. Clinical gynecologic endocrino. 1983: 1-41;
• Strasburger CJ, Kirschbaum C, Rohde W, Dressendorfer RA, Stahl F. Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement. J Steroid Biochem Mol Biol. 1992 Dec;43 (7):683-92

By research topic:

• Steroid hormones

By molecule:

• Estrone

Registration form