Estrone Human ELISA (Multispecies specificity)

Research topic

Animal studies, Steroid hormones

Summary

Estrone is a steroid like estriol and estradiol, belonging to the class of estrogens. The estrogens are involved in the development of female sex organs and secondary sex characteristics. Before the ovum is fertilized the main action of the estrogens is on the growth and function of the reproductive tract in order to prepare it for the fertilized ovum. During the follicular phase of the menstrual cycle the estrone level shows a slight increase.

Assay format

Competitive ELISA, Immobilized antigen

Sample requirements

50 μl

Applications

Serum

Calibration range

0 – 2000 pg/ml

Limit of detection

10.0 pg/ml

Intra-assay

CV = 7.7%

Inter-assay

CV = 9.5%

Spiking Recovery

99.45%

Dilution Linearity

95.5%