Ferritin Rat ELISA

Summary

Ferritin, molecular weight 450 000, is iron storage protein containing 24 units of 2 different subunits at various ratios. It contains 4 500 iron atoms in a molecule. Ferritin exist in spleen, liver, red marrow, small intestinal mucosa and muscle, and involve in iron metabolism of inner or outer cellular. Ferritin is a source of supply for metallic iron, and has a role to store iron, which catalyzes the hydroxy-radical synthesize reaction, into core of protein molecule. Determination of ferritin in serum is useful for diagnostic of disorder in iron metabolism, hepatic disease and malignant tumor.

Research topic

Animal studies, Iron metabolism

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample requirements

50 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

3.13 to 100 ng/ml

References to summary

• Bolton AE, Lee-Own V, McLean RK, Challand GS. Three different radioiodination methods for human spleen ferritin compared. Clin Chem. 1979 Oct;25 (10):1826-30
• Deinard AS, Sundberg RD, Lamb CM, Gonyea LM. Comparison of three procedures for isolating human ferritin, for use as a standard in an immunoradiometric assay. Clin Chem. 1976 Apr;22 (4):513-8
• Drysdale JW. Ferritin as a Tumor Marker. J. Clin. Immunoassay. 6/3:234-240., 1983¨;
• Linpisarn S, Kricka LJ, Kennedy JH, Whitehead TP. Sensitive sandwich enzyme immunoassay for serum ferritin on microtitre plates. Ann Clin Biochem. 1981 Jan;18 (Pt 1):48-53
• Pelletier C, Ali AM, Gauldie J, Walker WH, Luxton AW. A radioimmunoassay for serum ferritin. Clin Chem. 1977;23 (4):683-9
• Thomas MJ, Goldie DJ. Measurement of serum ferritin by radioimmunoassay. Ann Clin Biochem. 1978 Mar;15 (2):102-8
• Valdes R Jr, Brown BA, Ng RH. Three commercial methods for serum ferritin compared and the high-dose "hook effect" eliminated. Clin Chem. 1983 Jun;29 (6):1109-13
• Vernon AP, et al. Ferritin: A Reliable Assay for Detection of Iron Deficiency. The Ligand Quarterly. 3/4:15-16, 1980;

By research topic:

• Animal studies
• Iron metabolism

By molecule:

• Ferritin