Ferritin Rat ELISA

Research topic

Animal studies, Iron metabolism

Storage/Shipping

+4°C/ Wet ice

Summary

Ferritin, molecular weight 450 000, is iron storage protein containing 24 units of 2 different subunits at various ratios. It contains 4 500 iron atoms in a molecule. Ferritin exist in spleen, liver, red marrow, small intestinal mucosa and muscle, and involve in iron metabolism of inner or outer cellular. Ferritin is a source of supply for metallic iron, and has a role to store iron, which catalyzes the hydroxy-radical synthesize reaction, into core of protein molecule. Determination of ferritin in serum is useful for diagnostic of disorder in iron metabolism, hepatic disease and malignant tumor.

Assay format

Sandwich ELISA, HRP-labelled antibody

Sample requirements

50 µl/well

Applications

Serum

Calibration Curve

Calibration range

3.13 to 100 ng/ml