Fibronectin Human ELISA
| Other names: | Cold insoluble globulin, FINC, Large external transformation sensitive protein, Migration stimulating factor, Fibronectin precursor | ||
|---|---|---|---|
| Cat. No.: |
 RBMS2028R |
Regulatory status: RUO | |
| Size: | 96 wells (1 kit) | | | |
| Files: | Datasheet PDF (RUO) | ||
| Legend: | new product discount |
 temporarily out of stock order in advance |
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Notice
Restriction of a minimal quantity of 5 kits per order may apply.
Research topic
Cardiovascular disease
Storage/Shipping
Store kit reagents between 2 – 8°C.
Summary
Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis. Fibronectin is secreted as a dimer with a monomer molecular weight of 220–250 kDa. It is synthesized by, and present around, fibroblasts, endothelial cells, chondrocytes, glial cells, and myocytes. Fibronectin´s importance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation.
Assay format
Sandwich ELISA, Biotin-labelled antibody
Sample requirements
50 µl
Applications
Cell culture supernatant, Serum
Calibration Curve
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Calibration range
0.31 – 20 ng/ml
Limit of detection
0.1 ng/ml
Limit of quantification
2000 ng/ml (serum, plasma), 0.2 ng/ml (urine)
Intra-assay
CV = 5.3%
Inter-assay
CV = 6.7%
Spiking Recovery
110.6%
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