Fibronectin Human ELISA

Summary

Fibronectin is an ubiquitous extracellular glycoprotein that exists in a soluble form in body fluids and in an insoluble form in the extracellular matrix. It plays a major role in many important physiological processes, such as embryogenesis, wound healing, hemostasis and thrombosis. Fibronectin is secreted as a dimer with a monomer molecular weight of 220–250 kDa. It is synthesized by, and present around, fibroblasts, endothelial cells, chondrocytes, glial cells, and myocytes. Fibronectin´s im­portance during cancer progression has further been shown. It represents an active element in the process of T cell activation in the immune cascade triggered by organ transplantation.

Research topic

Oncology, Transplantation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma-EDTA, Plasma-Heparin, Serum, Urine

Sample requirements

50 µl

Storage/Shipping

Store kit reagents between 2 – 8°C.

Calibration Curve

Calibration range

0.31 – 20 ng/ml

Limit of detection

0.1 ng/ml

Intra-assay

CV = 5.3%

Inter-assay

CV = 6.7%

Spiking Recovery

110.6%

By research topic:

• Oncology
• Transplantation

By molecule:

• Fibronectin

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