FSH Rat ELISA

Summary

FSH is a glycoprotein with molecular weight about 35kDa, and is a heterodimer composed of α-subunit (a common subunit to FSH, LH and TSH) and specific β-subunit. FSH is produced by basophilic cells (gonadotrophs) of the pituitary together with LH. FSH acts on ovarian follicle in females to cause follicular growth, estrogen production and secretion, and on testicular seminal tubules causing growth and sperm production with the help of androgen in males. FSH secretion is stimulated by a hypothalamic peptide, GnRH. Another stimulatory substance is activin produced in pituitary, ovary, placenta, hypothalamus, etc. FSH biosynthesis is inhibited by inhibin, and follistatin also inhibits FSH synthesis through inactivation of activin. Inhibiting action of PACAP (pitiutary adenylatecyclase activating peptide) on FSH production is made by promotion of follistatin production. Sex steroid hormones lower FSH synthesis and secretion via hypothalamus (negative feedback), and may directly act on FSH gene. Circulating FSH levels change with sexual cycle, and increase after menopause, aging, gonadectomy, and azospermia, while stay low in infancy, pregnancy and confinement.

Features

• Highly sensitive assay with the standard range of 0.4 ~ 20 ng/ml.
• This kit is for FSH in rat serum or plasma (use EDTA-2Na for anticoagulant at the final concentration of 1 mg/ml).
• Assay sample volume is 20 µl in the standard procedure.
• Assay format is 96 wells.
• Standard preparation is rat origin.
• Components of the kit are provided ready to use or in concentrated form.

Research topic

Reproduction

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma, Serum

Sample requirements

20 µl/well

Storage/Shipping

When the complete kit is stored at 2 – 8°C, the kit is stable until the expiration date shown on the label on the box. Opened reagents should be used as soon as possible to avoid less than optimal assay performance caused by storage environment.

Calibration Curve

Calibration range

0.4 to 20 ng/ml

Limit of detection

40 pg/ml

By research topic:

• Reproduction

By molecule:

• FSH