GAD Autoantibody Human ELISA

Summary

Autoantibodies to pancreatic beta cell antigens are important serological markers of type 1 diabetes mellitus (type 1 DM). The antigens recognised by these antibodies include insulin, glutamic acid decarboxylase (GAD65 kDa isoform) and an islet cell antigen IA-2 or ICA-512.

Research topic

Autoimmunity, Diabetology - Autoimmunity

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum

Sample requirements

100 μl

Storage/Shipping

• 20°C

Calibration Curve

Calibration range

0 – 2000 U/ml

Limit of detection

0.06 U/ml

Intra-assay

CV = 7.9%

Inter-assay

CV = 5.5%

References to summary

• Brooking H, Ananieva-Jordanova R, Arnold C, Amoroso M, Powell M, Betterle C, Zanchetta R, Furmaniak J, Smith BR. A sensitive non-isotopic assay for GAD65 autoantibodies. Clin Chim Acta. 2003 May;331 (1-2):55-9
• Chen S, Willis J, Maclean C, Ananieva-Jordanova R, Amoroso MA, Brooking H, Powell M, Collins A, Bennett S, Mitchell S, Burne P, Furmaniak J, Smith BR. Sensitive non-isotopic assays for autoantibodies to IA-2 and to a combination of both IA-2 and GAD65. Clin Chim Acta. 2005 Jul 1;357 (1):74-83

By research topic:

• Autoimmunity
• Diabetology - Autoimmunity

By molecule:

• GAD Autoantibody