GDF-15/MIC-1 Human ELISA

Summary

GDF-15/MIC-1 is synthesized as a 62-kDa precursor protein, which, after cleavage by furin-like protease, is secreted as 25-kDa disulfide-linked dimmer. GDF-15/MIC-1 is weakly produced under baseline condition in most tissues such as brain, liver, kidney, pancreas, but not normally in many other organs including the heart, and is highly expressed in placenta and moderately in prostate. GDF-15 is upregulated by cardiovascular events triggering oxidative stress, including pressure overload, and atherosclerosis. Moreover, increased circulating GDF-15 concentrations have been linked to an enhanced risk of future adverse cardiovascular events in elderly woman and it was describe as a new biomarker of the risk of death in patients with non-ST-elevation acute coronary syndrome. Serum GDF-15 concentrations increase in maternal serum with advancing gestation in normal pregnancy. Low GDF-15 concentrations reportedly are associated with an increased risk of preterm labor or miscarriage.

Features

• It is intended for research use only.
• The total assay time is less than 3.5 hours.
• The kit measures Human GDF-15 in serum, plasma (EDTA, citrate, heparin).
• Assay format is 96 wells.
• Quality Controls are human serum based. No animal sera are used.
• Standard is recombinant protein based.
• Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Cardiovascular disease, Oncology, Reproduction

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma, Serum

Sample requirements

30 μl

Storage/Shipping

Store the complete kit at 2 – 8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration range

70 – 4480 pg/ml

Limit of detection

22 pg/ml

Intra-assay (Within-Run, n=8)

CV = 6.8%

Inter-assay (Run-to-Run, n=8)

CV = 9.0%

Spiking Recovery

96.3%

Dilution Linearity

93.4%

Cross-Reactivity

Monkey

References to this product

• Stejskal D, Karpisek M, Humenanska V, Lacnak B, Svestak M. Macrophage-inhibitory cytokine-1 (mic-1) in differential diagnosis of dyspnea-A pilot study. Clin Biochem. 2009 Mar 31;

References to summary

• Wollert KC, Kempf T, Lagerqvist B, Lindahl B, Olofsson S, Allhoff T, Peter T, Siegbahn A, Venge P, Drexler H, Wallentin L. Growth differentiation factor 15 for risk stratification and selection of an invasive treatment strategy in non ST-elevation acute coronary syndrome. Circulation. 2007 Oct 2;116 (14):1540-8
• Kempf T, Bjorklund E, Olofsson S, Lindahl B, Allhoff T, Peter T, Tongers J, Wollert KC, Wallentin L. Growth-differentiation factor-15 improves risk stratification in ST-segment elevation myocardial infarction. Eur Heart J. 2007 Dec;28 (23):2858-65

By research topic:

• Cardiovascular disease
• Oncology
• Reproduction

By molecule:

• GDF-15/MIC-1