Ghrelin Acylated Rat ELISA

Summary

Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.

Features

• The kit measures plasma acylated ghrelin.
• Special blood collection procedure is needed.
• Quality controls are rat-plasma based.

Research topic

Animal studies, Energy metabolism and body weight regulation

Assay format

Sandwich ELISA, AChE-labelled antibody

Applications

Plasma-EDTA+PHMB

Sample requirements

20 µl/well

Storage/Shipping

Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Curve

Calibration range

1.96 – 250 pg/ml

Limit of detection

The Limit of Determination, calculated as the concentration of acylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.2 pg/ml and 0.7 pg/ml for long and short first incubation period, respectively.

Intra-assay

CV = 8.2 % (20h at +4°C)
CV = 5.5 % (3h at RT)

Inter-assay

CV = 8.5 % (20h at +4°C)
CV = 6.5 % (3h at RT)

Spiking Recovery

99.0 % (20h at +4°C)
99.8 % (3h at RT)

Dilution Linearity

104.6 %

Cross-Reactivity

Human

By research topic:

• Animal studies
• Energy metabolism and body weight regulation

By molecule:

• Ghrelin