Cat # changed from RSCYK090R to YK090
Type
Competitive ELISA
Description
This EIA kit i s used for quantitative determination of rat, mouse or human pancreatic glucagon in
plasma samples. T he kit is characterized by sensitive quantification and high specificity. In addition , it is not influence by other components in plasma samples and sample pre treatment is unnecessary.
Glucagon standard used in kit system is highly purified synthetic product (purity: higher than 98%)
and biotinylated pancreatic glucagon is purified by HPLC.
Applications
Plasma
Sample Requirements
50 µl or 100 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
41–10 000 pg/ml
Intra-assay (Within-Run)
3.3-5.1 %
Inter-assay (Run-to-Run)
7.3-18.9 %
Spiking Recovery
101-110 %
Specificity
The EIA kit has high specificity to pancreatic glucagon and shows no cross reactivity with intestinal
glucagon, GLP 1 and GLP 2.
Features
- RUO
- test sample: plasma (rat, mouse,human)
- assay range 41-10000 pg/mL
- the assay duration change according the sample volume: 100 μL (20-24 hr.+1.5 hr.), 50 μL ( 44-48 hr.+1.5 hr)
- with one assay kit, 41 samples can be measured in duplicate
- intraassay CV = 3.3-5.1%
- interassay CV = 7.3 18.9%
Research topic
Diabetology - Other Relevant Products, Animal studies
Summary
According to many studies on glucagon immunoassay, it has been established that the antibody against the C terminal fragment (19-29) of glucagon has specific bin ding with pancreatic glucagon, whereas the antibody against the N terminal fragment 1-19) of glucagon has specific binding with both of pancreatic and intestinal glucagon (total glucagon). Once, 30K by Unger et. al had been widely used as an antibody spe cific for the C terminal fragment of glucagon, but Nishino, Shima and Yanaihara et. al succeeded in producing pancreatic glucagon specific antibody using synthetic peptide with the C terminal fragment (19-29) of glucagon as immunogen in 1981.
This EIA kit has been developed by using polyclonal antibody against glucagons (19-29), synthetic pancreatic glucagon as gl ucagon standard and biotinylated pancreatic glucagon as labeled antigen for the measurement of rat, mouse or human glucagon in plasma.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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