GM-CSF Rat ELISA

Summary

GM-CSF is a small glycoprotein growth factor which stimulates the production and function of neutrophils, eosinophils and monocytes. It can be produced by a wide variety of tissue types. The biological effects of GM-CSF are mediated through its binding to cell surface receptors, which appear to be widely expressed by thermatopoietic cells and also by some non-thermatopoietic cells, such as endothelial cells.

Research topic

Animal studies, Cytokines and chemokines and related molecules

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

2–8°C

Calibration Curve

Calibration range

7.8 – 500 pg/ml

Limit of detection

3.5 pg/ml

Intra-assay

CV = 5.0%

Inter-assay

CV = 0,1

Spiking Recovery

85%

Dilution Linearity

108 %

By research topic:

• Animal studies
• Cytokines and chemokines and related molecules

By molecule:

• GM-CSF