Granzyme A Human ELISA

Summary

Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes and NK cells. Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders (viral infections, lymphomas, carcinomas, rheumatoid arthritis and transplantation).

Research topic

Apoptosis, Immune Response, Infection and Inflammation, Transplantation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

Store standard and Biotin-Conjugate or whole kit at –20°C. The standard and Biotin-Conjugate can also be removed, stored at  –20°C, remaining kit reagents stored at 2–8°C.

Calibration Curve

Calibration range

0.8 – 600 pg/ml

Limit of detection

0.4 pg/ml

Intra-assay

CV = 8.7%

Inter-assay

CV = 8.6%

Spiking Recovery

78%

Dilution Linearity

93%

References to summary

• Hamann D, Baars PA, Rep MH, Hooibrink B, Kerkhof-Garde SR, Klein MR, van Lier RA. Phenotypic and functional separation of memory and effector human CD8+ T cells. J Exp Med. 1997 Nov 3;186 (9):1407-18
• Spaeny-Dekking EH, Hanna WL, Wolbink AM, Wever PC, Kummer AJ, Swaak AJ, Middeldorp JM, Huisman HG, Froelich CJ, Hack CE. Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160 (7):3610-6
• Van Der Vliet HJ, Wolbink AM, Spaeny LH, Van Diepen FN, Froelich CJ, ten Berge IJ, Hack CE, Wever PC. The CD8+ granzyme B+ T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells. Immunology. 1998 Mar;93 (3):383-9

By research topic:

• Apoptosis
• Immune Response, Infection and Inflammation
• Transplantation

By molecule:

• Granzyme A

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