Granzyme A Human ELISA

Summary

Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes and NK cells. Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders (viral infections, lymphomas, carcinomas, rheumatoid arthritis and transplantation).

Research topic

Apoptosis, Immune Response, Infection and Inflammation, Transplantation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum

Sample requirements

50 µl

Storage/Shipping

Store standard and Biotin-Conjugate or whole kit at –20°C. The standard and Biotin-Conjugate can also be removed, stored at  –20°C, remaining kit reagents stored at 2–8°C.

Calibration Curve

Calibration range

0.8 – 600 pg/ml

Limit of detection

0.4 pg/ml

Intra-assay

CV = 8.7%

Inter-assay

CV = 8.6%

Spiking Recovery

78%

Dilution Linearity

93%

References to summary

• Hack CE, Froelich CJ, Huisman HG, Middeldorp JM, Swaak AJ, Kummer AJ, Wever PC, Wolbink AM, Hanna WL, Spaeny-Dekking EH. Extracellular granzymes A and B in humans: detection of native species during CTL responses in vitro and in vivo. J Immunol. 1998 Apr 1;160 (7):3610-6
• van Lier RA, Klein MR, Kerkhof-Garde SR, Hooibrink B, Rep MH, Baars PA, Hamann D. Phenotypic and functional separation of memory and effector human CD8+ T cells. J Exp Med. 1997 Nov 3;186 (9):1407-18
• Wever PC, Hack CE, ten Berge IJ, Froelich CJ, Van Diepen FN, Spaeny LH, Wolbink AM, Van Der Vliet HJ. The CD8+ granzyme B+ T-cell subset in peripheral blood from healthy individuals contains activated and apoptosis-prone cells. Immunology. 1998 Mar;93 (3):383-9

By research topic:

• Apoptosis
• Immune Response, Infection and Inflammation
• Transplantation

By molecule:

• Granzyme A

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