Granzyme B Human ELISA

Summary

Granzymes are exogenous serine proteinases (enzymes) that are released from cytoplasmic granules of cytotoxic lymphocytes and NK cells. Percentages of the Granzyme A and B positive CTLs can be determined by flow cytometry and immunocytochemical methods for many disorders (viral infections, lymphomas, carcinomas, rheumatoid arthritis and transplantation).

Research topic

Apoptosis, Immune Response, Infection and Inflammation, Transplantation

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Cell culture supernatant, Serum

Sample requirements

50 µl

Storage/Shipping

–20°C

Calibration Curve

Calibration range

0.7 – 480 pg/ml

Limit of detection

0.2 pg/ml

Intra-assay

CV = 8.5%

Inter-assay

CV = 10.4%

Spiking Recovery

117%

Dilution Linearity

88%

By research topic:

• Apoptosis
• Immune Response, Infection and Inflammation
• Transplantation

By molecule:

• Granzyme B

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