Growth Hormone Sensitive Human ELISA

Summary

Human growth hormone (hGH) is the product of the GH-1 gene located on chromosome 17 and expressed in pituitary cells. Bioactivity of GH is regulated by a specific binding protein formed by the extra cellular part of the cellular transmembran GH-receptor. In children and adolescent the hGH system is the main regulator of growth. It has a lipolytic effect too. GH deficiency shows disease pattern, equivalent to advanced aging.

Research topic

Growth hormone and factor-related products

Assay format

Sandwich ELISA, Biotin-labelled antibody

Applications

Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

100 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

50 to 1 000 pg/ml

Limit of detection

1.66 pg/ml

References to summary

• Baumann G, Amburn K, Drobny EC. Circadian variation of basal plasma growth hormone in man. J Clin Endocrinol Metab. 1983 Sep;57 (3):524-8
• Beck P, Schalch DS, Parker ML, Kipnis DM, Daughaday WH. Correlative studies of growth hormone and insulin plasma concentrations with metabolic abnormalities in acromegaly. J Lab Clin Med. 1965 Sep;66 (3):366-79
• Crowley S, Hindmarsh PC, Matthews DR, Brook CG. Growth and the growth hormone axis in prepubertal children with asthma. J Pediatr. 1995 Feb;126 (2):297-303
• Daughaday WH. The adenohypophysis In: "Textbook of Endocrinology", R.H. Williams ed., W.B. Saunders. 1981;Philadelphia, p;73
• Daughaday WH, Cryer PE. Growth hormone hypersecretion and acromegaly. Hosp Pract. 1978 Aug;13 (8):75-80
• Frasier SD. A preview of growth hormone stimulation tests in children. Pediatrics. 1974 Jun;53 (6):929-37
• Goldfine ID. Medical treatment of acromegaly. Annu Rev Med. 1978;29:407-15
• Hashida S, Nakagawa K, Ishikawa E, Ohtaki S. Basal level of human growth hormone (hGH) in normal serum. Clin Chim Acta. 1985 Sep 30;151 (2):185-6
• Ho KK, Leung KC, Hoffman DM, Reutens AT. Evaluation and application of a highly sensitive assay for serum growth hormone (GH) in the study of adult GH deficiency. J Clin Endocrinol Metab. 1995 Feb;80 (2):480-5
• Ho KK, Sernia C, O'Sullivan AJ, Rajkovic IA, Kelly JJ. Effects of different oral oestrogen formulations on insulin-like growth factor-I, growth hormone and growth hormone binding protein in post-menopausal women. Clin Endocrinol (Oxf). 1993 Nov;39 (5):561-7
• LI CH. Human growth hormone: perspective on its chemistry and physiology. In: "Advances in human growth hormone research", S. Raiti ed. U.S. Department of Health, Education and Welfare Publ. DMEW publ. no. (NJH). 74-612, p. 321;
• Massara F, Camanni F, Molinatti GM, Belforte L, Rosatello A. Effect of dopamine on plasma growth hormone and prolactin levels in normal and acromegalic subjects. J Clin Endocrinol Metab. 1977 Mar;44 (3):465-73
• Sherman BM, Wert RM Jr, Chen AB, Celniker AC. Variability in the quantitation of circulating growth hormone using commercial immunoassays. J Clin Endocrinol Metab. 1989 Feb;68 (2):469-76
• Thompson JQ, McMurry JF Jr, Ibarra JD Jr, Gilliland PF, Eddy RL. Human growth hormone release. Comparison of provocative test procedures. Am J Med. 1974 Feb;56 (2):179-85
• VanderLaan EF, Sigel MB, Tutwiler GF, Singh RN, Lewis UJ, VanderLaan WP. Human growth hormone: a complex of proteins. Recent Prog Horm Res. 1980;36:477-508

By research topic:

• Growth hormone and factor-related products

By molecule:

• Growth Hormone