hsCRP Canine ELISA

Summary

CRP is an acute-phase protein, produced exclusively in the liver. Serum concentration of CRP increases significantly in cases of both infectious and noninfectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours. CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatic arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease. The CRP level increases dramatically following microbial infections, and this can be particularly helpful for the diagnosis and monitoring of bacterial septicemia in neonates and other immunocompromised patients at risk.

Research topic

Animal studies, Immune Response, Infection and Inflammation, Sepsis

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample requirements

5 µl/well

Storage/Shipping

+4°C/ Wet ice

Calibration Curve

Calibration range

1.5 to 40.0 µg/ml

Limit of detection

3.6 ng/ml

Intra-assay

CV = 3.3 %

Inter-assay

CV = 2.3 %

References to summary

• Burton, S.A., et. al.: C-reactive protein concentration in dogs with inflammatory leukograms.Am. J.Vet. Res. 1994; 55:613 – 618.
• Casals, C. et. al.: Increase of C-reactive protein and decrease of surfactant protein A in surfactant after lung transplantati­on.Am. J. Respir. Crit. Care. Med. 1998; 157: 43 – 49.
• Conner, J.G., et. al.: Acute phase response in the dog following surgical trauma.Res. Vet. Sci. 1988; 45: 107 – 110.
• Eckersall, P.D. et. al.: An immunoturbidimetric assay for canine C-reactive protein.Vet. Res. Commun. 1991; 15: 17 – 24.
• Lindback, S., et. al.: The value of C-reactive protein as a marker of bacterial infection in patients with septicemia, endocarditis, and influenza.Scand. J. Infect. Dis. 1989; 21: 543 – 549.
• Ndung’u, J.M. et. al.: Elevation of the concentration of acute phase proteins in dogs infected with Trypanosoma brucei.Acta. Trop. 1991;49: 77 – 86.
• Otabe, K.: Physiological levels of C-reactive protein in normal canine sera.Vet. Res. Commun. 1998; 22: 77 – 85.
• Rikihisa, Y. et. al.: C-reactive protein and alpha1-acid glycoprotein levels in dogs infected with Ehrlichia canis.J. Clin. Microbiol. 1994; 32: 912 – 917.
• Yamamoto, S. et. al.:Determination of C-reactive protein in serum and plasma from healthy dogs and dogs with pneumonia by ELISA and slide reversed passive latex agglutination test.Vet. Q. 1994; 16: 74 – 77.
• Yamashita,K., et.al.: Canine acute phase response: relationship between serum cytokine activity and acute phase protein in dogs.J. Vet. Med. Sci. 1994; 56: 487 – 492.

By research topic:

• Animal studies
• Immune Response, Infection and Inflammation
• Sepsis

By molecule:

• CRP

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