hsCRP Mouse ELISA

Summary

CRP is an acute-phase protein, produced exclusively in the liver. Serum concentration of CRP increases significantly in cases of both infectious and noninfectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours. CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatic arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease. The CRP level increases dramatically following microbial infections, and this can be particularly helpful for the diagnosis and monitoring of bacterial septicemia in neonates and other immunocompromised patients at risk.

Research topic

Animal studies, Immune Response, Infection and Inflammation, Sepsis

Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Fluids, Plasma-Citrate, Plasma-EDTA, Plasma-Heparin, Serum

Sample requirements

5µl/well

Storage/Shipping

C-reactive protein kit components should be stored at 2–8°C. Bring them to room temperature (20–25°C) before opening bottles and plate pouches. Diluted conjugate remaining after use should be discarded. TMB substrate and stop solution are also stable at room temperature.

Calibration Curve

Calibration range

1.56 to 25.0 ng/ml

Limit of detection

2.5 ng/ml

Intra-assay (Within-Run, n=12)

CV = 4 %

Inter-assay (Run-to-Run, n=12)

CV = 6 %

By research topic:

• Animal studies
• Immune Response, Infection and Inflammation
• Sepsis

By molecule:

• CRP

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