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hsCRP Rat ELISA

Other names: C-Reactive Protein Product of BioVendor
Product: Size:
RH951CRP01R (regulatory status: RUO) 96 wells (1 kit)
Files: Datasheet PDF (RUO)MSDS (RUO) CRP on pubmed

Product details


Summary

CRP is an acute-phase protein, produced exclusively in the liver. Serum concentration of CRP increases significantly in cases of both infectious and noninfectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours. CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatic arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease. The CRP level increases dramatically following microbial infections, and this can be particularly helpful for the diagnosis and monitoring of bacterial septicemia in neonates and other immunocompromised patients at risk.

Research topic

Animal studies, Immune Response, Infection and Inflammation, Sepsis


Assay format

Sandwich ELISA, HRP-labelled antibody

Applications

Cell culture supernatant, Plasma, Serum, Synovial fluid, Tissue extract, Urine

Sample requirements

5 µl/well

Storage/Shipping

C-reactive protein kit components should be stored at 2–8°C. Bring them to room temperature (20–25°C) before opening bottles and plate pouches. Diluted conjugate remaining after use should be discarded. TMB substrate and stop solution are also stable at room temperature.

Calibration Curve

Calibration range

1.6–133.3 ng/ml

Limit of detection

2.5 ng/ml

Intra-assay (Within-Run, n=12)

CV = 5.2%

Inter-assay (Run-to-Run, n=12)

CV = 5.2%


References to summary

  • Buduneli E, Buduneli N, Vardar-Sengul S, Kardesler L, Atilla G, Lappin D, Kinane DF. Systemic low-dose doxycycline and alendronate administration and serum interleukin-1beta, osteocalcin, and C-reactive protein levels in rats. J Periodontol. 2005 Nov;76 (11):1927-33
  • Cai X, Wong YF, Zhou H, Xie Y, Liu ZQ, Jiang ZH, Bian ZX, Xu HX, Liu L. The comparative study of Sprague-Dawley and Lewis rats in adjuvant-induced arthritis. Naunyn Schmiedebergs Arch Phar. 2006 May;373 (2):140-7
  • Chen K, Li F, Li J, Cai H, Strom S, Bisello A, Kelley DE, Friedman-Einat M, Skibinski GA, McCrory MA, Szalai AJ, Zhao AZ. Induction of leptin resistance through direct interaction of C-reactive protein with leptin. Nat Med. 2006 Apr;12 (4):425-32
  • Cho WC, Yip TT, Chung WS, Leung AW, Cheng CH, Yue KK. Differential expression of proteins in kidney, eye, aorta, and serum of diabetic and non-diabetic rats. J Cell Biochem. 2006 Sep 1;99 (1):256-68
  • Kalani R, Judge S, Carter C, Pahor M, Leeuwenburgh C. Effects of caloric restriction and exercise on age-related, chronic inflammation assessed by C-reactive protein and interleukin-6. J Gerontol A Biol Sci Med Sci. 2006 Mar;61 (3):211-7
  • Ling S, Jamali F. Effect of early phase adjuvant arthritis on hepatic P450 enzymes and pharmacokinetics of verapamil: an alternative approach to the use of an animal model of inflammation for pharmacokinetic studies. Drug Metab Dispos. 2005 Apr;33 (4):579-86
  • Ortiz RM, Mamalis A, Navar LG. Aldosterone Receptor Antagonism Reduces Urinary C-Reactive Protein Excretion in Angiotensin II-Infused, Hypertensive Rats. J Am Soc Hypertens. 2009 May-Jun;3 (3):184-91
  • Shagdarsuren E, Wellner M, Braesen JH, Park JK, Fiebeler A, Henke N, Dechend R, Gratze P, Luft FC, Muller DN. Complement activation in angiotensin II-induced organ damage. Circ Res. 2005 Sep 30;97 (7):716-24

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